Speract induces calcium oscillations in the sperm tail.
Speract induces calcium oscillations in the sperm tail.
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DOI：
10.1083/jcb.200212053
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发表时间：
2003-04-14
期刊：
影响因子：
--
通讯作者：
Whitaker M
中科院分区：
文献类型：
--
作者：
Wood CD;Darszon A;Whitaker M
海胆精子活力受精子激活肽调节。一种这样的肽，speract，诱导细胞内游离钙浓度（[Ca 2 +]i）的变化。单精子高分辨率成像显示精子诱导的[Ca 2 +]i变化具有复杂的时空结构。[Ca2[Ca ~（2+）]i增加出现在尾部作为周期性振荡; [Ca ~（2+）]i增加在精子头部滞后于那些在尾部，似乎是由于尾部信号转导事件的总和。时间长短取决于精子浓度。在未受刺激的精子尾部也观察到罕见的自发[Ca 2 +]i瞬变，头部再次落后于尾部。Speract诱导的波动对膜电位和钙通道阻滞剂敏感，并被阴离子通道阻滞剂尼氟灭酸增强。3-异丁基-1-甲基黄嘌呤可增强cGMP/cAMP信号通路，消除尾部的[Ca 2 +]i波动，导致头部[Ca 2 +]i非常延迟和持续的增加。这些数据指向了一个模型，其中尾部周期性产生的信使扩散到头部。精子是高度极化的细胞。我们的研究结果表明，一个清晰的理解之间的联系[Ca 2 +]i和精子活力将只能通过分析的[Ca 2 +]i信号的水平上的单个精子。
Sea urchin sperm motility is modulated by sperm-activating peptides. One such peptide, speract, induces changes in intracellular free calcium concentration ([Ca2+]i). High resolution imaging of single sperm reveals that speract-induced changes in [Ca2+]i have a complex spatiotemporal structure. [Ca2+]i increases arise in the tail as periodic oscillations; [Ca2+]i increases in the sperm head lag those in the tail and appear to result from the summation of the tail signal transduction events. The period depends on speract concentration. Infrequent spontaneous [Ca2+]i transients were also seen in the tail of unstimulated sperm, again with the head lagging the tail. Speract-induced fluctuations were sensitive to membrane potential and calcium channel blockers, and were potentiated by niflumic acid, an anion channel blocker. 3-isobutyl-1-methylxanthine, which potentiates the cGMP/cAMP-signaling pathways, abolished the [Ca2+]i fluctuations in the tail, leading to a very delayed and sustained [Ca2+]i increase in the head. These data point to a model in which a messenger generated periodically in the tail diffuses to the head. Sperm are highly polarized cells. Our results indicate that a clear understanding of the link between [Ca2+]i and sperm motility will only be gained by analysis of [Ca2+]i signals at the level of the single sperm.
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