Spatial single-cell sequencing of meiosis I arrested oocytes indicates acquisition of maternal transcripts from the soma.
Spatial single-cell sequencing of meiosis I arrested oocytes indicates acquisition of maternal transcripts from the soma.
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DOI：
10.1016/j.celrep.2023.112544
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发表时间：
2023-06-27
期刊：
影响因子：
8.8
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中科院分区：
文献类型：
--
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母体RNA在整个减数分裂I停滞期间以转录静止状态储存在卵母细胞中数分钟至数十年。然而，最近的报告提出了一个作用，新生转录被捕卵母细胞。被捕的卵母细胞是否启动新生转录响应环境或激素信号，同时保持减数分裂I逮捕仍然不确定。我们通过整合单细胞RNA测序、RNA速率和RNA荧光原位杂交技术对C.减数分裂I阻滞卵母细胞。我们确定的成绩单，增加作为逮捕减数分裂I卵母细胞的年龄，但排除细胞外信号通过ERK MAPK和新生转录作为这种增加的机制。我们报告转录收购邻近体细胞作为一种机制的转录增加减数分裂I逮捕。这些分析提供了一个更深层次的见解，在单细胞分辨率的RNA景观的减数分裂我逮捕卵母细胞，因为它准备卵母细胞成熟和受精。母体转录物，这是重要的早期胚胎发生，都被认为是起源于生殖细胞谱系。Trimmer等人表明，一些独特的转录本在体细胞中产生并加载到卵母细胞中，这表明母体转录本的其他来源。
Maternal RNAs are stored from minutes to decades in oocytes throughout meiosis I arrest in a transcriptionally quiescent state. Recent reports, however, propose a role for nascent transcription in arrested oocytes. Whether arrested oocytes launch nascent transcription in response to environmental or hormonal signals while maintaining the meiosis I arrest remains undetermined. We test this by integrating single-cell RNA sequencing, RNA velocity, and RNA fluorescence in situ hybridization on C. elegans meiosis I arrested oocytes. We identify transcripts that increase as the arrested meiosis I oocyte ages, but rule out extracellular signaling through ERK MAPK and nascent transcription as a mechanism for this increase. We report transcript acquisition from neighboring somatic cells as a mechanism of transcript increase during meiosis I arrest. These analyses provide a deeper view at single-cell resolution of the RNA landscape of a meiosis I arrested oocyte and as it prepares for oocyte maturation and fertilization. Maternal transcripts, which are important for early embryogenesis, are all thought to originate exclusively from the germ cell lineage. Trimmer et al. show that a few unique transcripts are produced in the somatic cells and loaded into oocytes, suggesting other sources of maternal transcripts.
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