Pathogenicity of Shiga Toxin Type 2e Escherichia coli in Pig Colibacillosis.
Pathogenicity of Shiga Toxin Type 2e Escherichia coli in Pig Colibacillosis.
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志贺毒素2e型大肠杆菌对猪大肠杆菌病的致病性。
DOI：
10.3389/fvets.2020.545818
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发表时间：
2020
影响因子：
3.2
通讯作者：
Alborali GL
中科院分区：
文献类型：
--
作者：
Baldo V;Salogni C;Giovannini S;D'Incau M;Boniotti MB;Birbes L;Pitozzi A;Formenti N;Grassi A;Pasquali P;Alborali GL
滋贺毒素2 e型（Stx 2 e）大肠杆菌是引起猪腹泻和水肿的致病因子。本研究的目的是确定产Stx 2 e E.大肠杆菌分离物，并对来自猪大肠杆菌病临床病例和健康猪的分离物进行表征。在11年的研究期间（2006-2017），共检测到233株Stx 2 e菌株，其中2，060株中有230株（11.16%）。健康猪171头中检出3头（1.75%）。产Stx 2 e的分离株在临床大肠杆菌病病例中确实比在健康猪中存在更多（p = 0.0002）。优势血清群为O 139（79.82%），菌毛因子以F18（177株）最常见，其次为F6（5株）。肠毒素LTI、STa和STb的检出率分别为10.43%、41.73%和48.26%。F18-Stx 2 e和-STa-STb-Stx 2 e在断奶仔猪和育肥猪中的检出率相似（分别为33.33%和35.52%和38.30%和25.53%）。在来自病猪的分离物中，O 139和F18分别是最常见的血清群和毒力因子。230株Stx 2 e阳性菌株中，有29株（12.60%）携带ESBL基因，其中TEM（79.30%）、CTX-M1（17.20%）和CMY-2（3.40%）最多。对四环素耐药率最高（98.25%），其次为氨苄西林（93.91%）、头孢菌素（90.43%）和甲氧苄啶/磺胺甲恶唑（82.17%）。结果表明，产Stx 2 e的E.大肠杆菌更频繁地与大肠杆菌病的临床形式相关，从健康猪中分离这些分离株的可能性极小。
Shiga toxin type 2e (Stx2e) Escherichia coli is the causative factor of diarrhea and edema in swine. The aims of this study were to determine the prevalence of Stx2e-producing E. coli isolates and to characterize isolates from clinical cases of pig colibacillosis and healthy swine. During the 11 years of the study (2006–2017), a total of 233 Stx2e-producing isolates were detected−230 out of 2,060 (11.16%) E. coli isolated from diseased pigs and 3 out of 171 (1.75%) from healthy swine. Stx2e-producing isolates were indeed more present in clinical colibacillosis cases than in healthy pigs (p = 0.0002). The predominant serogroup was O139 (79.82%) and the most common fimbrial factor present in these isolates was F18 (177 isolates), followed by F6 (5 isolates). The enterotoxins LTI, STa, and STb were detected in 10.43, 41.73, and 48.26% of the isolates, respectively. The predominant virotypes F18-Stx2e and -STa-STb-Stx2e were similarly present in weaners (33.33 and 35.52%) and finishers (38.30 and 25.53%). Among isolates from diseased pigs, O139 and F18 were the more frequently identified serogroup and virulence factor, respectively. Of the tested 230 Stx2e-producing isolates isolated from diseased pigs, 29 (12.60%) harbored genes encoding ESBL, particularly TEM (79.30%), CTX-M1 (17.20%), and CMY-2 (3.40%). Antimicrobial resistance to tetracycline was the most common characteristic (98.25%), followed by ampicillin (93.91%), cephalotin (90.43%) and trimethoprim/sulfamethoxazole (82.17%). Our results showed that Stx2e-producing E. coli were more frequently associated with clinical forms of colibacillosis, with minimal probability to isolate these isolates from healthy pigs.
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通讯作者：
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影响因子：
9.4
作者：
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Bosworth, Brad T.
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作者：
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Feder I;Wallace FM;Gray JT;Fratamico P;Fedorka-Cray PJ;Pearce RA;Call JE;Perrine R;Luchansky JB
通讯作者：
Luchansky JB