Comparison of the effects of buffalo LIF and mouse LIF on the in vitro culture of buffalo spermatogonia
Comparison of the effects of buffalo LIF and mouse LIF on the in vitro culture of buffalo spermatogonia
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水牛LIF与小鼠LIF对水牛精原细胞体外培养效果的比较
DOI：
10.1002/cbin.11994
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发表时间：
2023-01
影响因子：
3.9
通讯作者：
Xiao Gan Yang
中科院分区：
文献类型：
--
作者：
Ya Ru Liu;Wang Chang Li;Jia Hao Hu;Qi Qi Li;Ya Ping Zhang;Ke Huan Lu;Hui Yan Xu;Xing Wei Liang;Yang Qing Lu;Xiao Gan Yang
白血病抑制因子（LIF）是一种重要的生长因子，通过抑制自发分化支持精原干细胞（SSCs）的培养和维持。不同的LIF序列可能导致功能上的差异。MEGA软件分析表明，布法罗LIF与小鼠LIF的蛋白质序列差异达65.5%。构建PB-LIF-GFP-Puro载体，建立CHO-K1细胞系。CHO‐K1细胞培养基中的最终LIF蛋白浓度约为4.268 ng/mL。在这里，我们报告说，布法罗LIF有效地保持自我更新的布法罗精原细胞在培养过程中。将布法罗精原细胞培养在不含LIF（0 ng/mL）、小鼠LIF（1 ng/mL）、小鼠LIF（10 ng/mL）或布法罗LIF（1 ng/mL）的条件培养基中。此外，通过分析细胞集落形成、定量真实的时间聚合酶链反应、细胞免疫荧光和细胞计数来确定小鼠LIF和布法罗LIF培养物对布法罗精原细胞维持的影响。布法罗LIF（1 ng/mL）组对布法罗精原细胞增殖的维持与小鼠LIF（10 ng/mL）组相似。这些结果表明，低剂量的布法罗LIF可以维持布法罗精原细胞的体外增殖。本研究为进一步优化布法罗SSC体外培养体系奠定了基础。
Leukemia inhibitory factor (LIF) is an important growth factor that supports the culture and maintenance of spermatogonial stem cells (SSCs) by suppressing spontaneous differentiation. Different LIF sequences may lead to differences in function. The protein sequences of buffalo LIF and mouse LIF differed by 65.5% according to MEGA software analysis. The PB‐LIF‐GFP‐Puro vector was constructed, and the CHO‐K1 cell line was established. The final LIF protein concentration in the CHO‐K1 cell culture medium was approximately 4.268 ng/mL. Here, we report that buffalo LIF effectively maintains the self‐renewal of buffalo spermatogonia during culture. Buffalo spermatogonia were cultured in conditioned medium containing no LIF (0 ng/mL), mouse LIF (1 ng/mL), mouse LIF (10 ng/mL), or buffalo LIF (1 ng/mL). Furthermore, the effects of mouse LIF and buffalo LIF culture on the maintenance of buffalo spermatogonia were determined by analyzing cell colony formation, quantitative real‐time polymerase chain reaction, cell immunofluorescence, and cell counting. The buffalo LIF (1 ng/mL) group showed similar maintenance of the proliferation of buffalo spermatogonia to that in the mouse LIF (10 ng/mL) group. These results demonstrated that the proliferation of buffalo spermatogonia can be maintained in vitro by adding a low dose of buffalo LIF. This study provides a foundation for the further optimization of in vitro buffalo SSC culture systems.
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影响因子：
--
作者：
Benjamin L. Kidder
通讯作者：
Benjamin L. Kidder
Wnt/β-catenin和LIF-Stat3信号通路汇聚在Sp5上促进小鼠胚胎干细胞自我更新
影响因子：
4
作者：
Ye S;Zhang D;Cheng F;Wilson D;Mackay J;He K;Ban Q;Lv F;Huang S;Liu D;Ying QL
通讯作者：
Ying QL
影响因子：
5.8
作者：
Codino A;Turowski T;van de Lagemaat LN;Ivanova I;Tavosanis A;Much C;Auchynnikava T;Vasiliauskaitė L;Morgan M;Rappsilber J;Allshire RC;Kranc KR;Tollervey D;O'Carroll D
通讯作者：
O'Carroll D
影响因子：
5.6
作者：
Dong, Lihua;Gul, Murat;Andersen, Claus Yding
通讯作者：
Andersen, Claus Yding
影响因子：
64.5
作者：
Ying, QL;Nichols, J;Smith, A
通讯作者：
Smith, A