Metagenomes of tropical soil-derived anaerobic switchgrass-adapted consortia with and without iron.
Metagenomes of tropical soil-derived anaerobic switchgrass-adapted consortia with and without iron.
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DOI：
10.4056/sigs.3377516
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发表时间：
2013
影响因子：
--
通讯作者：
Hazen TC
中科院分区：
文献类型：
--
作者：
Deangelis KM;D'Haeseleer P;Chivian D;Simmons B;Arkin AP;Mavromatis K;Malfatti S;Tringe S;Hazen TC
Tropical forest soils decompose litter rapidly with frequent episodes of anoxia, making it likely that bacteria using alternate terminal electron acceptors (TEAs) such as iron play a large role in supporting decomposition under these conditions. The prevalence of many types of metabolism in litter deconstruction makes these soils useful templates for improving biofuel production. To investigate how iron availability affects decomposition, we cultivated feedstock-adapted consortia (FACs) derived from iron-rich tropical forest soils accustomed to experiencing frequent episodes of anaerobic conditions and frequently fluctuating redox. One consortium was propagated under fermenting conditions, with switchgrass as the sole carbon source in minimal media (SG only FACs), and the other consortium was treated the same way but received poorly crystalline iron as an additional terminal electron acceptor (SG + Fe FACs). We sequenced the metagenomes of both consortia to a depth of about 150 Mb each, resulting in a coverage of 26× for the more diverse SG + Fe FACs, and 81× for the relatively less diverse SG only FACs. Both consortia were able to quickly grow on switchgrass, and the iron-amended consortium exhibited significantly higher microbial diversity than the unamended consortium. We found evidence of higher stress in the unamended FACs and increased sugar transport and utilization in the iron-amended FACs. This work provides metagenomic evidence that supplementation of alternative TEAs may improve feedstock deconstruction in biofuel production.
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影响因子：
3
作者：
Edgar RC
通讯作者：
Edgar RC
影响因子：
3.6
作者：
DeAngelis, Kristen M.;Gladden, John M.;Hazen, Terry C.
通讯作者：
Hazen, Terry C.
影响因子：
11
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Barberan, Albert;Bates, Scott T.;Fierer, Noah
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Fierer, Noah
影响因子：
14.9
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Finn RD;Mistry J;Tate J;Coggill P;Heger A;Pollington JE;Gavin OL;Gunasekaran P;Ceric G;Forslund K;Holm L;Sonnhammer EL;Eddy SR;Bateman A
通讯作者：
Bateman A
影响因子：
3.7
作者：
DeAngelis KM;Allgaier M;Chavarria Y;Fortney JL;Hugenholtz P;Simmons B;Sublette K;Silver WL;Hazen TC
通讯作者：
Hazen TC