Regulation of coronafacoyl phytotoxin production by the PAS-LuxR family regulator CfaR in the common scab pathogen Streptomyces scabies.
Regulation of coronafacoyl phytotoxin production by the PAS-LuxR family regulator CfaR in the common scab pathogen Streptomyces scabies.
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DOI：
10.1371/journal.pone.0122450
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Bignell DR
中科院分区：
文献类型：
--
作者：
Cheng Z;Bown L;Tahlan K;Bignell DR
马铃薯疮痂病是一种以马铃薯块茎表面形成浅的、隆起的或凹陷的病斑为特征的重要经济作物病害。该疾病分布最广的病原体是疥疮链霉菌，其产生植物毒性次级代谢物thaxtomin A，该代谢物是该生物体的关键毒力因子。最近的研究表明，S.疥疮还可产生植物毒性次级代谢物冠脂酰-L-异亮氨酸（CFA-L-Ile）以及少量的其它相关代谢物。用于产生CFA-L-Ile的生物合成基因的表达依赖于PAS-LuxR家族转录调节因子CfaR，其在S.疥疮在这项研究中，我们表明，CfaR激活coronafacoyl植物毒素的生产，通过结合到一个单一的网站位于上游的推定的-35六核苷酸盒内的启动子区域的生物合成基因。CfaR的结合活性需要LuxR和PAS结构域，后者参与蛋白质同源二聚体的形成。我们还表明，在S.通过cfaR和下游共转录基因orf 1的过度表达来治疗疥疮。我们的研究结果提供了重要的洞察冠脂酰植物毒素的生产，这被认为是有助于毒力表型的S。疥疮此外，我们提供的证据表明，CfaR是PAS-LuxR家族的调节剂，其成员广泛分布于放线菌细菌中的一个新成员。
Potato common scab is an economically important crop disease that is characterized by the formation of superficial, raised or pitted lesions on the potato tuber surface. The most widely distributed causative agent of the disease is Streptomyces scabies, which produces the phytotoxic secondary metabolite thaxtomin A that serves as a key virulence factor for the organism. Recently, it was demonstrated that S. scabies can also produce the phytotoxic secondary metabolite coronafacoyl-L-isoleucine (CFA-L-Ile) as well as other related metabolites in minor amounts. The expression of the biosynthetic genes for CFA-L-Ile production is dependent on a PAS-LuxR family transcriptional regulator, CfaR, which is encoded within the phytotoxin biosynthetic gene cluster in S. scabies. In this study, we show that CfaR activates coronafacoyl phytotoxin production by binding to a single site located immediately upstream of the putative -35 hexanucleotide box within the promoter region for the biosynthetic genes. The binding activity of CfaR was shown to require both the LuxR and PAS domains, the latter of which is involved in protein homodimer formation. We also show that CFA-L-Ile production is greatly enhanced in S. scabies by overexpression of both cfaR and a downstream co-transcribed gene, orf1. Our results provide important insight into the regulation of coronafacoyl phytotoxin production, which is thought to contribute to the virulence phenotype of S. scabies. Furthermore, we provide evidence that CfaR is a novel member of the PAS-LuxR family of regulators, members of which are widely distributed among actinomycete bacteria.
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影响因子：
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作者：
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