IL-21 modulates release of proinflammatory cytokines in LPS-stimulated macrophages through distinct signaling pathways.
IL-21 modulates release of proinflammatory cytokines in LPS-stimulated macrophages through distinct signaling pathways.
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IL-21 通过不同的信号通路调节 LPS 刺激的巨噬细胞中促炎细胞因子的释放
DOI：
10.1155/2013/548073
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发表时间：
2013
影响因子：
4.6
通讯作者：
Liu JX
中科院分区：
文献类型：
--
作者：
Li SN;Wang W;Fu SP;Wang JF;Liu HM;Xie SS;Liu BR;Li Y;Lv QK;Li ZQ;Xue WJ;Huang BX;Chen W;Liu JX
本研究旨在探讨IL-21对lps诱导的小鼠腹腔巨噬细胞的抗炎作用。结果表明，IL-21显著抑制lps诱导的巨噬细胞IL-1β、TNF-α和IL-6 mRNA的表达，但对IFN-γ、IL-10、CCL5和CXCL2的mRNA表达无明显抑制作用。ELISA分析显示，IL-21还能抑制lps诱导的培养上清中TNF-α和IL-6的产生。Western blot分析显示，IL-21明显抑制lps刺激的巨噬细胞ERK和i -κB α磷酸化和NF-κB易位，但增加STAT3磷酸化。流式细胞术和Western blot分析显示，IL-21降低了lps刺激细胞中M1巨噬细胞表面标志物CD86、iNOS和TLR4的表达。所有结果表明，IL-21通过抑制ERK的磷酸化和NF-κB的易位来减少IL-6和TNF-α的产生，并通过降低CD86、iNOS和TLR4的表达以及增加lps刺激细胞中STAT3的磷酸化来促进巨噬细胞从M1向M2表型的转变。
The aim of this study was to investigate the anti-inflammatory effect of IL-21 on LPS-induced mouse peritoneal macrophages. The results showed that IL-21 significantly inhibited LPS-induced mRNA expression of IL-1β, TNF-α, and IL-6 in macrophages, but not of IFN-γ, IL-10, CCL5, or CXCL2. ELISA analysis showed that IL-21 also suppressed LPS-induced production of TNF-α and IL-6 in culture supernatants. Western blot analysis showed that IL-21 clearly inhibited ERK and IκBα phosphorylation and NF-κB translocation in LPS-stimulated macrophages, but it increased STAT3 phosphorylation. Flow cytometric and Western blot analysis showed that IL-21 decreased M1 macrophages surface markers expression of CD86, iNOS, and TLR4 in LPS-stimulated cells. All results suggested that IL-21 decreases IL-6 and TNF-α production via inhibiting the phosphorylation of ERK and translocation of NF-κB and promotes a shift from the M1 to M2 macrophage phenotype by decreasing the expression of CD86, iNOS, and TLR4 and by increasing STAT3 phosphorylation in LPS-stimulated cells.
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姜黄素消除了LPS诱导的264.7巨噬细胞中的促炎细胞因子。涉及SOCS -1，-3和P38 MAPK的新型机制的证据。
影响因子：
3
作者：
Guimaraes, Morgana Rodrigues;Manzoli Leite, Fabio Renato;Spolidorio, Luis Carlos;Kirkwood, Keith Lough;Rossa, Carlos, Jr.
通讯作者：
Rossa, Carlos, Jr.
影响因子：
11.2
作者：
Li SH;Hawthorne VS;Neal CL;Sanghera S;Xu J;Yang J;Guo H;Steeg PS;Yu D
通讯作者：
Yu D
影响因子：
4.4
作者：
Caruso, Roberta;Fina, Daniele;Monteleone, Giovanni
通讯作者：
Monteleone, Giovanni
影响因子：
64.8
作者：
Foster, Simmie L.;Hargreaves, Diana C.;Medzhitov, Ruslan
通讯作者：
Medzhitov, Ruslan
DOI：
10.1007/978-1-62703-523-1_12
发表时间：
2013
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Mo, Jinyao;Duncan, Joseph A
通讯作者：
Duncan, Joseph A