Curcumin abrogates LPS-induced pro-inflammatory cytokines in RAW 264.7 macrophages. Evidence for novel mechanisms involving SOCS-1, -3 and p38 MAPK.
Curcumin abrogates LPS-induced pro-inflammatory cytokines in RAW 264.7 macrophages. Evidence for novel mechanisms involving SOCS-1, -3 and p38 MAPK.
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姜黄素消除了LPS诱导的264.7巨噬细胞中的促炎细胞因子。涉及SOCS -1，-3和P38 MAPK的新型机制的证据。
DOI：
10.1016/j.archoralbio.2013.07.005
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发表时间：
2013-10
影响因子：
3
通讯作者：
Rossa, Carlos, Jr.
中科院分区：
文献类型：
--
作者：
Guimaraes, Morgana Rodrigues;Manzoli Leite, Fabio Renato;Spolidorio, Luis Carlos;Kirkwood, Keith Lough;Rossa, Carlos, Jr.
姜黄素是姜黄根茎提取物中的活性化合物，具有通过抑制细胞内信号传导介导的抗炎特性。SOCS和MAP激酶参与调控IL-6、TNF-α和PGE 2表达的信号转导过程，在慢性炎症性疾病中发挥重要作用。目的是评估这些途径是否参与姜黄素介导的对巨噬细胞中LPS诱导的这些细胞因子表达的影响。在存在和不存在非细胞毒性浓度的姜黄素的情况下，用大肠杆菌LPS刺激RAW 264.7鼠巨噬细胞。姜黄素可有效抑制LPS诱导的IL-6、TNF-α和考克斯-2 mRNA的表达，并通过调节p38 MAPK的核转位，阻止LPS诱导的SOCS-1和-3表达的抑制以及对p38 MAPK活化的抑制。总之，姜黄素通过涉及SOCS-1和SOCS-3以及p38 MAPK的表达和活性的调节的机制有效地抑制LPS诱导的巨噬细胞中的炎性细胞因子的表达。
Curcumin is the active compound in the extract of Curcuma longa rhizomes with anti-inflammatory properties mediated by inhibition of intracellular signalling. SOCS and MAPKinases are involved in the signalling events controlling the expression of IL-6, TNF-α and PGE2, which have important roles on chronic inflammatory diseases. The aim was to assess if these pathways are involved in curcumin-mediated effects on LPS-induced expression of these cytokines in macrophages. RAW 264.7 murine macrophages were stimulated with Escherichia coli LPS in the presence and absence of non-cytotoxic concentrations of curcumin. Curcumin potently inhibited LPS-induced expression of IL-6, TNF-α and COX-2 mRNA and prevented LPS-induced inhibition of SOCS-1 and -3 expression and the inhibition of the activation of p38 MAPKinase by modulation of its nuclear translocation. In conclusion, curcumin potently inhibits expression of LPS-induced inflammatory cytokines in macrophages via mechanisms that involve modulation of expression and activity of SOCS-1 and SOCS-3 and of p38 MAPK.
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影响因子：
4.4
作者：
Bharti, AC;Takada, Y;Aggarwal, BB
通讯作者：
Aggarwal, BB
DOI：
10.1073/pnas.88.12.5292
发表时间：
1991-06-01
影响因子：
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作者：
HUANG, TS;LEE, SC;LIN, JK
通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
3
作者：
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通讯作者：
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影响因子：
12.6
作者：
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