Chloroplast Signaling Gates Thermotolerance in Arabidopsis.
Chloroplast Signaling Gates Thermotolerance in Arabidopsis.
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DOI：
10.1016/j.celrep.2018.01.054
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发表时间：
2018-02-13
期刊：
影响因子：
8.8
通讯作者：
Wigge PA
中科院分区：
文献类型：
--
作者：
Dickinson PJ;Kumar M;Martinho C;Yoo SJ;Lan H;Artavanis G;Charoensawan V;Schöttler MA;Bock R;Jaeger KE;Wigge PA
温度是影响植物生长和生存的重要环境变量。真核生物对高温胁迫的保护是由热休克因子（HSFs）协调的，热休克因子是激活保护性伴侣如热休克蛋白70 （HSP70）表达的转录因子；然而，温度被感知并与其他环境信号整合成适应性反应的途径尚不清楚。植物暴露在相当大的温度日变化中，我们发现拟南芥的耐热性存在日变化，最大的耐热性与白天较高的HSP70表达一致。在正向遗传筛选中，我们发现叶绿体在控制这种反应中起关键作用，这表明光诱导的叶绿体信号在其中起关键作用。与此一致的是，我们能够通过改变植物中的氧化还原状态而独立于热休克来全局激活HSFA1a与其靶标的结合。拟南芥基础耐热性的日表达规律与热相关基因的日表达规律相关叶绿体突变体具有更强的热休克基因表达和耐热性。叶绿体产生光信号门HSFA1和热休克基因表达植物在白天对热胁迫的适应性最强，这一反应受激活热休克基因的HSFA1转录因子的控制。Dickinson等人发现叶绿体电子传递的扰动会影响热休克基因的表达。他们发现HSFA1的活性是由依赖光的叶绿体信号控制的。
Temperature is a key environmental variable influencing plant growth and survival. Protection against high temperature stress in eukaryotes is coordinated by heat shock factors (HSFs), transcription factors that activate the expression of protective chaperones such as HEAT SHOCK PROTEIN 70 (HSP70); however, the pathway by which temperature is sensed and integrated with other environmental signals into adaptive responses is not well understood. Plants are exposed to considerable diurnal variation in temperature, and we have found that there is diurnal variation in thermotolerance in Arabidopsis thaliana, with maximal thermotolerance coinciding with higher HSP70 expression during the day. In a forward genetic screen, we identified a key role for the chloroplast in controlling this response, suggesting that light-induced chloroplast signaling plays a key role. Consistent with this, we are able to globally activate binding of HSFA1a to its targets by altering redox status in planta independently of a heat shock. There is a diurnal pattern of basal thermotolerance in Arabidopsis Thermotolerance correlates with diurnal expression patterns of heat-associated genes Chloroplast mutants have greater heat shock gene expression and thermotolerance A chloroplast generated light signal gates HSFA1 and heat shock gene expression Plants are most resilient to heat stress during the day, a response controlled by HSFA1 transcription factors activating heat shock genes. Dickinson et al. find that perturbations of chloroplast electron transport affect heat shock gene expression. They show that HSFA1 activity is gated by a light-dependent chloroplast signal.
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DOI：
10.1073/pnas.94.25.14168
发表时间：
1997-12-09
影响因子：
11.1
作者：
Kropat, J;Oster, U;Beck, CF
通讯作者：
Beck, CF
DOI：
10.1126/science.89.2320.556-a
发表时间：
1939-06-16
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Laude, H H
通讯作者：
Laude, H H
DOI：
10.1016/s0044-328x(84)80082-3
发表时间：
1984-01-01
期刊：
ZEITSCHRIFT FUR PFLANZENPHYSIOLOGIE
影响因子：
--
作者：
KAPPEN, L;LOSCH, R
通讯作者：
LOSCH, R
影响因子：
6.9
作者：
Ogawa, Daisuke;Yamaguchi, Kazuo;Nishiuchi, Takumi
通讯作者：
Nishiuchi, Takumi
影响因子：
56.9
作者：
Niittylä, T;Messerli, G;Zeeman, SC
通讯作者：
Zeeman, SC