Experimental and Genomic Evaluation of the Oestrogen Degrading Bacterium Rhodococcus equi ATCC13557.
Experimental and Genomic Evaluation of the Oestrogen Degrading Bacterium Rhodococcus equi ATCC13557.
复制标题
DOI：
10.3389/fmicb.2021.670928
复制
发表时间：
2021
影响因子：
5.2
通讯作者：
Davenport RJ
中科院分区：
文献类型：
--
作者：
Harthern-Flint SL;Dolfing J;Mrozik W;Meynet P;Eland LE;Sim M;Davenport RJ
马红球菌ATCC13557是一种能够降解17-乙基雌二醇(17α-ehenylestediol，EE_2)的模型生物，可以作为研究雌激素降解的模型生物。生物降解实验表明，马乳杆菌ATCC13557不能代谢EE2。然而，它能够代谢E2，主要代谢产物是E1，不能进一步降解E1。然而，E_2向E_1的转化是不完全的，在混合(E_1-E_2-E_2)和仅E_2的条件下，分别有11.2%和50.6%的E_2被降解。因此，马乳杆菌ATCC13557降解E2的代谢途径可能有两条。首次对马乳杆菌ATCC13557的基因组进行了测序、组装和作图。通过基因组分析，可以确定可能与观察到的马立克氏杆菌ATCC13557的生物降解特性有关的基因。马乳杆菌ATCC13557中的几个基因与其他雌激素降解菌基因组中鉴定的基因相似，但在序列上不完全相同，包括恶臭假单胞菌SJTE-1和鞘氨醇单胞菌KC8。在马乳杆菌ATCC13557基因组中发现了编码可能参与雌激素降解的酶的同源基因序列，最常见的是细胞色素P450单加氧酶(OECB)、外源双加氧酶(OECC)和17β-羟基类固醇脱氢酶(OECA)。这些搜索还发现了一个基因簇，可能编码参与类固醇/雌激素降解的酶；3-羧乙基儿茶酚2，3-双加氧酶、2-羟基粘液酸半醛水解酶、3-α(或20-β)-羟基类固醇脱氢酶、3-(3-羟基苯基)丙酸羟基酶、细胞色素P450单加氧酶和3-氧类固醇1-脱氢酶。此外，搜索发现类固醇激素代谢基因簇来自9，10-SECO途径，因此马R.ATCC13557也具有代谢其他类固醇激素如胆固醇的潜力。
Rhodococcus equi ATCC13557 was selected as a model organism to study oestrogen degradation based on its previous ability to degrade 17α-ethinylestradiol (EE2). Biodegradation experiments revealed that R. equi ATCC13557 was unable to metabolise EE2. However, it was able to metabolise E2 with the major metabolite being E1 with no further degradation of E1. However, the conversion of E2 into E1 was incomplete, with 11.2 and 50.6% of E2 degraded in mixed (E1-E2-EE2) and E2-only conditions, respectively. Therefore, the metabolic pathway of E2 degradation by R. equi ATCC13557 may have two possible pathways. The genome of R. equi ATCC13557 was sequenced, assembled, and mapped for the first time. The genome analysis allowed the identification of genes possibly responsible for the observed biodegradation characteristics of R. equi ATCC13557. Several genes within R. equi ATCC13557 are similar, but not identical in sequence, to those identified within the genomes of other oestrogen degrading bacteria, including Pseudomonas putida strain SJTE-1 and Sphingomonas strain KC8. Homologous gene sequences coding for enzymes potentially involved in oestrogen degradation, most commonly a cytochrome P450 monooxygenase (oecB), extradiol dioxygenase (oecC), and 17β-hydroxysteroid dehydrogenase (oecA), were identified within the genome of R. equi ATCC13557. These searches also revealed a gene cluster potentially coding for enzymes involved in steroid/oestrogen degradation; 3-carboxyethylcatechol 2,3-dioxygenase, 2-hydroxymuconic semialdehyde hydrolase, 3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase, 3-(3-hydroxy-phenyl)propionate hydroxylase, cytochrome P450 monooxygenase, and 3-oxosteroid 1-dehydrogenase. Further, the searches revealed steroid hormone metabolism gene clusters from the 9, 10-seco pathway, therefore R. equi ATCC13557 also has the potential to metabolise other steroid hormones such as cholesterol.
登录
查看更多内容
影响因子：
9.8
作者：
Czajka, Cynthia P.;Londry, Kathleen L.
通讯作者：
Londry, Kathleen L.
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
影响因子：
14.9
作者：
Dereeper A;Guignon V;Blanc G;Audic S;Buffet S;Chevenet F;Dufayard JF;Guindon S;Lefort V;Lescot M;Claverie JM;Gascuel O
通讯作者：
Gascuel O
影响因子：
8.6
作者：
Chen, Yi-Lung;Yu, Chang-Ping;Chiang, Yin-Ru
通讯作者：
Chiang, Yin-Ru
影响因子：
3
作者：
Andaluri, Gangadhar;Suri, Rominder P. S.;Kumar, Kuldip
通讯作者：
Kumar, Kuldip