Effects of sulfate deprivation on the production of chondroitin/dermatan sulfate by cultures of skin fibroblasts from normal and diabetic individuals.
Effects of sulfate deprivation on the production of chondroitin/dermatan sulfate by cultures of skin fibroblasts from normal and diabetic individuals.
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缺乏硫酸盐对正常和糖尿病个体皮肤成纤维细胞培养物产生软骨素/硫酸皮肤素的影响。
DOI：
10.1016/0003-9861(91)90340-o
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发表时间：
1991
影响因子：
3.9
通讯作者：
Jeremiah E. Silbert
中科院分区：
文献类型：
--
作者：
Cynthia K. Silbert;Donald E. Humphries;Mary E. Palmer;Jeremiah E. Silbert
来自两名正常男性、三名I型糖尿病男性和一名I型糖尿病女性的人皮肤成纤维细胞单层培养在低浓度硫酸盐存在的情况下与[~3H]氨基葡萄糖孵育。虽然不同细胞系[~3H]软骨素/皮肤素糖胺聚糖的总合成略有不同，但当硫酸盐浓度从0.3 mM降至0.06 mM至0.01 mM至0时，任何细胞系内的糖胺聚糖的合成均不受影响。硫酸盐浓度的降低导致软骨素/皮肤胶的硫酸盐化逐渐减少，从而使其中一条线的总硫酸盐化降至19%。随着培养基中硫酸盐浓度的降低，软骨素的硫酸盐化成4-硫酸软骨素和6-硫酸软骨素的影响是递进的且相等的。然而，硫酸盐化形成的皮肤素硫酸盐在更大程度上被保存下来，从而使皮肤素硫酸盐与软骨素硫酸盐的相对比例增加。基本上所有的非硫化残基都对软骨素AC裂解酶敏感，这表明在没有硫化的情况下，葡萄糖醛酸残基几乎没有发生向艾杜糖酸的异构化。这些结果证实了先前所描述的葡萄糖醛酸/艾杜糖醛二糖异构化对硫酸的依赖性，并表明含有艾杜糖醛酸的皮胶二糖残基的硫酸盐浓度可以低于含有葡萄糖的软骨素二糖残基的6-硫酸盐和4-硫酸盐的硫酸盐浓度。6个成纤维细胞系对低硫酸盐的敏感性以及6-硫酸软骨素、4-硫酸软骨素和皮肤素的比例存在较大差异。然而，正常人和糖尿病患者之间并没有不同的模式。
Human skin fibroblast monolayer cultures from two normal men, three Type I diabetic men, and one Type I diabetic woman were incubated with [3H]glucosamine in the presence of diminished concentrations of sulfate. Although total synthesis of [3H]chondroitin/dermatan glycosaminoglycans varied somewhat between cell lines, glycosaminoglycan production was not affected within any line when sulfate levels were decreased from 0.3 mmto 0.06 mmto 0.01 mmto 0 added sulfate. Lowering of sulfate concentrations resulted in diminished sulfation of chondroitin/dermatan in a progressive manner, so that overall sulfation dropped to as low as 19% for one of the lines. Sulfation of chondroitin to form chondroitin 4-sulfate and chondroitin 6-sulfate was progressively and equally affected by decreasing the sulfate concentration in the culture medium. However, sulfation to form dermatan sulfate was preserved to a greater degree, so that the relative proportion of dermatan sulfate to chondroitin sulfate increased. Essentially all the nonsulfated residues were susceptible to chondroitin AC lyase, indicating that little epimerization of glucuronic acid residues to iduronic acid had occurred in the absence of sulfation. These results confirm the previously described dependency of glucuronic/iduronic epimerization on sulfation, and indicate that sulfation of the iduronic acid-containing disaccharide residues of dermatan can take place with sulfate concentrations lower than those needed for 6-sulfation and 4-sulfation of the glucuronic acid-containing disaccharide residues of chondroitin. There were considerable differences among the six fibroblast lines in susceptibility to low sulfate medium and in the proportion of chondroitin 6-sulfate, chondroitin 4-sulfate, and dermatan sulfate. However, there was no pattern of differences between normals and diabetics.
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正常成人皮肤成纤维细胞的六种品系之间蛋白聚糖合成的变异性。
影响因子：
2.9
作者：
Vogel,KG;Campbell,K
通讯作者：
Campbell,K
中国仓鼠卵巢细胞硫酸盐转运缺陷突变体。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Esko,JD;Elgavish,A;Prasthofer,T;Taylor,WH;Weinke,JL
通讯作者：
Weinke,JL
通过正常和糖尿病皮肤成纤维细胞培养物生产[3H]己糖胺标记的蛋白多糖：用来自葡萄糖和其他来源的内源性己糖胺稀释外源性[3H]葡萄糖胺。
影响因子：
3.9
作者：
Silbert,CK;Palmer,ME;Humphries,DE;Silbert,JE
通讯作者：
Silbert,JE
DOI：
10.1042/bj2520305
发表时间：
1988
期刊：
The Biochemical journal
影响因子：
--
作者：
Humphries,DE;Silbert,CK;Silbert,JE
通讯作者：
Silbert,JE