31P nuclear magnetic resonance measurements of muscle glucose-6-phosphate. Evidence for reduced insulin-dependent muscle glucose transport or phosphorylation activity in non-insulin-dependent diabetes mellitus.
31P nuclear magnetic resonance measurements of muscle glucose-6-phosphate. Evidence for reduced insulin-dependent muscle glucose transport or phosphorylation activity in non-insulin-dependent diabetes mellitus.
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肌肉葡萄糖-6-磷酸的 31P 核磁共振测量。
DOI：
10.1172/jci115686
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Shulman,GI
中科院分区：
文献类型：
--
作者：
Rothman,DL;Shulman,RG;Shulman,GI
为了评估非胰岛素依赖型糖尿病（NIDDM）肌糖原合成的限速步骤，在高血糖-高胰岛素钳夹过程中，通过31 P核磁共振（NMR）测量葡萄糖-6-磷酸（G6 P）的浓度。在胰岛素（约450 pmol/L）和葡萄糖（11 mmol/L）的稳态血浆浓度相似的情况下，对6名NIDDM受试者和6名年龄体重匹配的对照组进行了研究。通过31 P NMR测量腓肠肌中G6 P的浓度。通过胰岛素-葡萄糖钳夹技术结合间接量热法测定全身氧化和非氧化葡萄糖代谢。在这些条件下，非氧化葡萄糖代谢是肌糖原合成的量度（1990. N. Engl. J.Med.322：223-228），在正常受试者中为31 +/-7 mumol/（kg体重-分钟），在NIDDM受试者中为13 +/-3 mumol/（kg体重-分钟）（P小于0.05）。正常人G6 P浓度（0.24 ± 0.02 mmol/kg肌肉）高于NIDDM患者（0.17 ± 0.02，P <0.01）。在4名NIDDM受试者中，将胰岛素浓度增加至胰岛素8，500 pmol/L使葡萄糖摄取率和G6 P浓度恢复至正常水平。总之，尽管非氧化葡萄糖代谢速率降低，但糖尿病受试者中G6 P浓度较低，这与肌肉葡萄糖转运或磷酸化缺陷降低肌糖原合成速率一致。
To assess the rate-limiting step in muscle glycogen synthesis in non-insulin-dependent diabetes mellitus (NIDDM), the concentration of glucose-6-phosphate (G6P) was measured by 31P nuclear magnetic resonance (NMR) during a hyperglycemic-hyperinsulinemic clamp. Six subjects with NIDDM and six age weight-matched controls were studied at similar steady-state plasma concentrations of insulin (approximately 450 pmol/liter) and glucose (11 mmol/liter). The concentration of G6P in the gastrocnemius muscle was measured by 31P NMR. Whole-body oxidative and nonoxidative glucose metabolism was determined by the insulin-glucose clamp technique in conjunction with indirect calorimetry. Nonoxidative glucose metabolism which under these conditions is a measure of muscle glycogen synthesis (1990. N. Engl. J. Med. 322:223-228), was 31 +/- 7 mumol/(kg body wt-min) in the normal subjects and 13 +/- 3 mumol/(kg body wt-min) in the NIDDM subjects (P less than 0.05). The concentration of G6P was higher (0.24 +/- 0.02 mmol/kg muscle) in the normal subjects than in the NIDDM subjects (0.17 +/- 0.02, P less than 0.01). Increasing insulin concentrations to insulin 8,500 pmol/liter in four NIDDM subjects restored the glucose uptake rate and G6P concentrations to normal levels. In conclusion, the lower concentration of G6P in the diabetic subjects despite a decreased rate of nonoxidative glucose metabolism is consistent with a defect in muscle glucose transport or phosphorylation reducing the rate of muscle glycogen synthesis.
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影响因子：
158.5
作者：
SHULMAN, GI;ROTHMAN, DL;SHULMAN, RG
通讯作者：
SHULMAN, RG
局部磁共振。
影响因子：
4
作者：
R. E. Gordon
通讯作者：
R. E. Gordon
活组织中的 31P NMR：从有前途的生物学工具到重要的生物学工具的道路
DOI：
--
发表时间：
1979
期刊：
影响因子：
--
作者：
D. Gadian;G. Radda;R. Richards;P. Seeley
通讯作者：
P. Seeley
胰岛素抵抗 I 型糖尿病患者骨骼肌葡萄糖处理限速缺陷的定位
影响因子：
7.7
作者：
H. Yki;K. Sahlin;J. Ren;V. Koivisto
通讯作者：
V. Koivisto
人体骨骼肌中葡萄糖和胰岛素对糖原合成酶和磷酸化酶活性的调节。
DOI：
10.1172/jci113069
发表时间：
1987
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Hannele Yki;David M. Mott;Andrew A. Young;K. Stone;Clifton Bogardus
通讯作者：
Clifton Bogardus