Membrane potential measurements of transitional cells from the crista ampullaris of the Gerbil
Membrane potential measurements of transitional cells from the crista ampullaris of the Gerbil
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沙鼠壶腹嵴移行细胞的膜电位测量
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
D. C. Marcus
中科院分区：
文献类型：
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作者：
P. Wangemann;D. C. Marcus
用微电极刺穿壶腹嵴移行细胞，记录膜电位，研究膜的性质。对照溶液PD为- 87±1 mV （n=103）。该值与在无Cl−溶液中测量的- 83±2 mV （n=24）无显著差异。[Cl−]从150到15 mmol/l （n=24）使膜去极化约2 mV，表明有较小的Cl−电导。PD对K+在3.6 ~ 25 mmol/l范围内的响应得到的K+转移数为0.75±0.01 （n=79）。Ba2+（2·10−6 ~ 10−3 mol/l）、奎尼丁（10−3 mol/l）、奎宁（10−3 mol/l）、Rb+ (20 mmol/l)、Cs+ (20 mmol/l)、NH4+ (20 mmol/l)和Tl+ (0.5 mmol/l)降低了[K+]步骤的细胞膜去极化和PD反应幅度，而四乙基铵（TEA, 20 mmol/l）对[K+]步骤无影响。在3.6 mmol/l K+存在下，Ba2+的剂量-响应曲线在25 mmol/l K+存在下右移约30年，在135 mmol/l葡萄糖酸盐作为Cl−的替代品存在下右移约4倍。沃巴因使移行细胞去极化，提示存在（Na++K+- atp酶）。
Transitional cells of the crista ampullaris were impaled with microelectrodes in order to record the membrane potential (PD) and to investigate membrane properties. In control solution the PD was −87±1 mV (n=103). This value is not significantly different from −83±2 mV (n=24) measured in Cl− free solution. [Cl−] steps from 150 to 15 mmol/l (n=24) depolarized the membrane by about 2 mV, indicating a minor Cl− conductance. The transference number for K+ was 0.75±0.01 (n=79) obtained from the PD responses to K+ steps from 3.6 to 25 mmol/l. The cell membrane depolarized and the amplitude of PD responses to [K+] steps was reduced by Ba2+ (2·10−6 to 10−3 mol/l), quinidine (10−3 mol/l), quinine (10−3 mol/l), Rb+ (20 mmol/l), Cs+ (20 mmol/l), NH4+ (20 mmol/l) and Tl+ (0.5 mmol/l), whereas tetraethylammonium (TEA, 20 mmol/l) had no effect. The dose-response curve for Ba2+ in the presence of 3.6 mmol/l K+ was shifted to the right by approximately three decades in the presence of 25 mmol/l K+ and by a factor of about 4 in the presence of 135 mmol/l gluconate as a substitute for Cl−. Transitional cells were depolarized by ouabain, suggesting the presence of (Na++K+-ATPase.
培养的髓质厚升肢细胞中 Ca2 激活 K 通道的阻断剂。
DOI：
10.1152/ajpcell.1987.252.2.c128
发表时间：
1987
期刊：
The American journal of physiology
影响因子：
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作者：
Guggino,SE;Guggino,WB;Green,N;Sacktor,B
通讯作者：
Sacktor,B
在离体龟结肠上皮细胞中利多卡因和奎尼丁阻断单一钾通道。
DOI：
10.1152/ajpcell.1986.251.1.c85
发表时间：
1986
期刊：
The American journal of physiology
影响因子：
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作者：
Richards,NW;Dawson,DC
通讯作者：
Dawson,DC