A glial variant of the vesicular monoamine transporter is required to store histamine in the Drosophila visual system.
A glial variant of the vesicular monoamine transporter is required to store histamine in the Drosophila visual system.
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DOI：
10.1371/journal.pgen.1000245
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发表时间：
2008-11
期刊：
影响因子：
4.5
通讯作者：
Krantz DE
中科院分区：
文献类型：
--
作者：
Romero-Calderón R;Uhlenbrock G;Borycz J;Simon AF;Grygoruk A;Yee SK;Shyer A;Ackerson LC;Maidment NT;Meinertzhagen IA;Hovemann BT;Krantz DE
与其他单胺类神经递质不同，大脑组胺含量调节的机制尚不清楚。在哺乳动物中，囊泡单胺转运蛋白（VMATs）仅在神经元中表达，并介导组胺和其他单胺的储存。我们研究了黑腹果蝇的视觉系统，其中组胺是光感受器细胞释放的主要神经递质。我们在此报告果蝇VMAT （DVMAT-B）的一种新的mRNA剪接变体不是在神经元中表达，而是在果蝇视叶层的一小部分胶质细胞中表达。组胺含量因dVMAT突变而降低，但在胶质细胞中特异性表达dVMAT - b可部分恢复组胺含量。我们的研究结果表明，单胺转运蛋白在胶质细胞中的新作用可能与其他系统中的组胺稳态有关。神经元是大脑中负责传递信息的细胞，它们通过一种被称为神经递质的化学物质相互交流。单胺类神经递质包括多巴胺、血清素和组胺，它们都起着重要的生理作用。然而，与多巴胺和5 -羟色胺不同，人们对大脑中组胺含量的调节知之甚少。我们正在用黑腹果蝇来研究脑细胞中组胺的储存和释放。哺乳动物和昆虫都使用一类被称为转运蛋白的蛋白质来储存胺，但迄今为止，人们一直认为胺转运蛋白仅限于神经元。我们发现，促进果蝇视觉系统神经元功能的支持细胞或神经胶质含有一种新形式的单胺转运体。尽管这种蛋白的分布受到限制，但它是维持整个视觉系统正常水平的组胺所必需的。我们推测其他动物可能使用类似的策略来调节这种重要的神经递质的功能。
Unlike other monoamine neurotransmitters, the mechanism by which the brain's histamine content is regulated remains unclear. In mammals, vesicular monoamine transporters (VMATs) are expressed exclusively in neurons and mediate the storage of histamine and other monoamines. We have studied the visual system of Drosophila melanogaster in which histamine is the primary neurotransmitter released from photoreceptor cells. We report here that a novel mRNA splice variant of Drosophila VMAT (DVMAT-B) is expressed not in neurons but rather in a small subset of glia in the lamina of the fly's optic lobe. Histamine contents are reduced by mutation of dVMAT, but can be partially restored by specifically expressing DVMAT-B in glia. Our results suggest a novel role for a monoamine transporter in glia that may be relevant to histamine homeostasis in other systems. Neurons, the cells in the brain responsible for carrying information, communicate with each other using a class of chemicals known as neurotransmitters. One family of neurotransmitters, the monoamines, includes dopamine, serotonin, and histamine, all of which play major physiological roles. However, unlike dopamine and serotonin, the regulation of the brain's histamine content is poorly understood. We are using the fruitfly Drosophila melanogaster to study the storage and release of histamine from brain cells. Both mammals and insects use a class of proteins called transporters to store amines, but, to date, amine transporters have been thought to be restricted to neurons. We have found that the support cells, or glia, that facilitate the function of neurons in the fly's visual system contain a new form of monoamine transporter. Despite its circumscribed distribution, this protein is required to maintain normal levels of histamine throughout the visual system. We speculate that other animals may use a similar strategy to regulate the function of this important neurotransmitter.
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DOI：
10.1002/neu.20146
发表时间：
2005-09-05
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
Greer, CL;Grygoruk, A;Krantz, DE
通讯作者：
Krantz, DE
影响因子：
25
作者：
Arnth-Jensen, N;Jabaudon, D;Scanziani, M
通讯作者：
Scanziani, M
影响因子：
2.5
作者：
Borycz, JA;Borycz, J;Meinertzhagen, IA
通讯作者：
Meinertzhagen, IA
影响因子：
4.5
作者：
Eiden, LE;Schäfer, MKH;Schütz, B
通讯作者：
Schütz, B
影响因子：
64.5
作者：
Chaudhry, FA;Reimer, RJ;Edwards, RH
通讯作者：
Edwards, RH