Human endonuclease V as a repair enzyme for DNA deamination.
Human endonuclease V as a repair enzyme for DNA deamination.
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DOI：
10.1016/j.mrfmmm.2012.05.003
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发表时间：
2012-07-01
影响因子：
2.3
通讯作者：
Cao, Weiguo
中科院分区：
文献类型：
--
作者：
Mi, Rongjuan;Alford-Zappala, Maria;Kow, Yoke W.;Cunningham, Richard P.;Cao, Weiguo
人核酸内切酶V基因位于染色体17q25.3，编码一个282个氨基酸的蛋白质，与细菌核酸内切酶V的序列相同性约为30%。本研究报告的生化特性的人核酸内切酶V的脱氨基的基础病变的修复。使用可溶性蛋白融合硫氧还蛋白的N-末端，我们确定了人核酸内切酶V对脱氧肌苷（I），脱氧黄苷（X），脱氧氧黄苷（O）和脱氧尿苷（U）的DNA的修复活性。人核酸内切酶V对含脱氧次黄嘌呤的DNA最具活性，但对含脱氧黄嘌呤的DNA具有较小的活性。未检测到脱氧氧核苷和脱氧尿苷的内切酶活性。对含脱氧肌苷的DNA的酶切活性顺序为单链I > G/I > T/I > A/I > C/I。催化活性的偏好与这些含脱氧肌苷的DNA的结合亲和力相关。Mg ~（2+）和Mn ~（2+）、Ni ~（2+）、Co ~（2+）在很小程度上可以支持核酸内切酶活性。将人核酸内切酶V导入缺乏nfi、mug和ung基因的大肠杆菌细胞中，导致突变频率降低三倍。这是第一次报告的人核酸内切酶V的脱氨基碱基修复活性。核酸内切酶活性和脱氨基脱氧腺苷（脱氧肌苷）修复之间的关系进行了讨论。
The human endonuclease V gene is located in chromosome 17q25.3 and encodes a 282 amino acid protein that shares about 30% sequence identity with bacterial endonuclease V. This study reports biochemical properties of human endonuclease V with respect to repair of deaminated base lesions. Using soluble proteins fused to thioredoxin at the N-terminus, we determined repair activities of human endonuclease V on deoxyinosine (I)-, deoxyxanthosine (X)-, deoxyoxanosine (O)- and deoxyuridine (U)-containing DNA. Human endonuclease V is most active with deoxyinosine-containing DNA but with minor activity on deoxyxanthosine-containing DNA. Endonuclease activities on deoxyoxanosine and deoxyuridine were not detected. The endonuclease activity on deoxyinosine-containing DNA follows the order of single-stranded I > G/I > T/I > A/I > C/I. The preference of the catalytic activity correlates with the binding affinity of these deoxyinosine-containing DNAs. Mg2+ and to a much less extent, Mn2+, Ni2+, Co2+ can support the endonuclease activity. Introduction of human endonuclease V into Escherichia coli cells deficient in nfi, mug and ung genes caused three-fold reduction in mutation frequency. This is the first report of deaminated base repair activity for human endonuclease V. The relationship between the endonuclease activity and deaminated deoxyadenosine (deoxyinosine) repair is discussed.
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影响因子：
14.9
作者：
Gao H;Huang J;Barany F;Cao W
通讯作者：
Cao W
影响因子：
14.9
作者：
MARTIN, FH;CASTRO, MM;TINOCO, I
通讯作者：
TINOCO, I
影响因子：
8
作者：
Huang, JM;Kirk, B;Barany, F
通讯作者：
Barany, F
DOI：
10.1016/s0921-8777(99)00063-4
发表时间：
2000-03-20
期刊：
MUTATION RESEARCH-DNA REPAIR
影响因子：
--
作者：
He, B;Qing, H;Kow, YW
通讯作者：
Kow, YW
影响因子：
4.8
作者：
Dou, H;Mitra, S;Hazra, TK
通讯作者：
Hazra, TK