Polycationic triazine-based dendrimers: effect of peripheral groups on transfection efficiency.
Polycationic triazine-based dendrimers: effect of peripheral groups on transfection efficiency.
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DOI：
10.1039/b908735d
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Simanek EE
中科院分区：
文献类型：
--
作者：
Mintzer MA;Merkel OM;Kissel T;Simanek EE
合成了一组由8个不同数量的胺、胍、羟基和外围脂肪基团组成的第二代三嗪树枝状大分子，并对其进行了基因转移测试，试图关联表面官能度对转染率的影响。分析了树枝状大分子和树枝状复合体的物理化学和生物学特性，如DNA的缩合、树枝状复合体的大小、表面电荷和形态、毒性以及最终的MeWo细胞转染率。溴化乙锭排斥实验的结果表明，树枝状大分子与DNA的络合效率受表面基团的影响中等。增加表面胺的数量，减少表面羟基的数量，或用胍类取代胺部分，都有助于加强形成的络合物。动态光散射和Zeta电位分析的结果表明，最小的颗粒与表现出最高Zeta电位的络合物相关。所有化合物的细胞毒性都很低，尤其是外围含有烷基的G2-5树状大分子，这表明将这种中性官能团结合到三嗪树状大分子的表面是有好处的。在这个小组中，观察到形成最小复合体的树枝状大分子的转染率最高，这表明这种物理化学性质是确定哪些树枝状大分子将表现出高转染率的准确预测指标。
A panel of eight, second generation triazine dendrimers differing in the number of amines, guanidines, hydroxyls and aliphatic groups on the periphery was synthesized and assayed for gene transfer in an attempt to correlate the effects of surface functionality on transfection efficiency. The physicochemical and biological properties of the dendrimers and dendriplexes, such as condensation of DNA, size, surface charge and morphology of dendriplexes, toxicity and ultimately transfection efficiency in MeWo cells, were analyzed. The results from an ethidium bromide exclusion assay showed that the complexation efficiency of the dendrimers with DNA is moderately affected by surface groups. Increasing the number of surface amines, reducing the number of surface hydroxyl groups, or replacing the amine moiety with guanidines all help strengthen the complex formed. Results from dynamic light scattering and zeta potential analyses indicate that the smallest particles correlate with complexes that exhibit the highest zeta potentials. Cytotoxicity was low for all compounds, particularly for the G2-5 dendrimer containing alkyl groups on the periphery, indicating the benefit of incorporating such neutral functionality onto the surface of the triazine dendrimers. Within this panel, the highest transfection efficiency was observed for the dendrimers that formed the smallest complexes, suggesting that this physicochemical property is an accurate predictor for determining which dendrimers will show high transfection efficiency.
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影响因子：
14
作者：
Kim, Tae-il;Baek, Jung-un;Park, Jong-sang
通讯作者：
Park, Jong-sang
影响因子：
14
作者：
Okuda, T;Sugiyama, A;Aoyagi, H
通讯作者：
Aoyagi, H
DOI：
10.1007/4-431-27879-6_8
发表时间：
2005-01-01
期刊：
NON-VIRAL GENE THERAPY: GENE DESIGN AND DELIVERY
影响因子：
--
作者：
Niidome, T;Katayama, Y
通讯作者：
Katayama, Y
用于癌细胞基因传递的 M-PEIs 纳米凝胶的尺寸依赖性特性
影响因子：
5.8
作者：
Xu, Dong-Mei;Yao, Si-De;Dong, Li
通讯作者：
Dong, Li
影响因子：
10.8
作者：
Choi, JS;Nam, K;Park, J
通讯作者：
Park, J