Reporter mRNAs cleaved by Rnt1p are exported and degraded in the cytoplasm.
Reporter mRNAs cleaved by Rnt1p are exported and degraded in the cytoplasm.
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DOI：
10.1093/nar/gkr627
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发表时间：
2011-11
影响因子：
14.9
通讯作者：
van Hoof A
中科院分区：
文献类型：
--
作者：
Meaux S;Lavoie M;Gagnon J;Abou Elela S;van Hoof A
对于大多数蛋白质编码基因，RNA聚合酶II的转录终止之前是新生转录物的核酸内切裂解。这种切割的3′产物通过5′核糖核酸外切酶Rat 1 p迅速降解，Rat 1 p被认为使RNA聚合酶II复合物不稳定。目前尚不清楚RNA切割是否足以触发核RNA降解和转录终止，或者RNA的命运是否取决于其他元件。对于大多数mRNA，这种切割是由切割和多聚腺苷酸化机制介导的，但它也可以由Rnt 1 p介导。我们发现，Rnt 1 p切割的mRNA是不足以触发核降解或转录终止。将Rnt 1 p靶位点插入到报告mRNA中并不阻断切割位点下游的转录，而是产生两种不稳定的切割产物，这两种产物都不能通过Rat 1 p的失活而稳定。相反，3′和5′切割产物通过细胞质5′核糖核酸外切酶（Xrn 1 p）的缺失或细胞质RNA外泌体的失活而稳定。这些数据表明，转录终止和核降解不是切割RNA的默认命运，并且需要特异性启动子和/或序列元件来确定切割产物的命运。
For most protein coding genes, termination of transcription by RNA polymerase II is preceded by an endonucleolytic cleavage of the nascent transcript. The 3′ product of this cleavage is rapidly degraded via the 5′ exoribonuclease Rat1p which is thought to destabilize the RNA polymerase II complex. It is not clear whether RNA cleavage is sufficient to trigger nuclear RNA degradation and transcription termination or whether the fate of the RNA depends on additional elements. For most mRNAs, this cleavage is mediated by the cleavage and polyadenylation machinery, but it can also be mediated by Rnt1p. We show that Rnt1p cleavage of an mRNA is not sufficient to trigger nuclear degradation or transcription termination. Insertion of an Rnt1p target site into a reporter mRNA did not block transcription downstream of the cleavage site, but instead produced two unstable cleavage products, neither of which were stabilized by inactivation of Rat1p. In contrast, the 3′ and 5′ cleavage products were stabilized by the deletion of the cytoplasmic 5′ exoribonuclease (Xrn1p) or by inactivation of the cytoplasmic RNA exosome. These data indicate that transcription termination and nuclear degradation is not the default fate of cleaved RNAs and that specific promoter and/or sequence elements are required to determine the fate of the cleavage products.
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影响因子：
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