New T cell epitopes identified from an anti-idiotypic antibody mimicking ovarian cancer associated antigen
New T cell epitopes identified from an anti-idiotypic antibody mimicking ovarian cancer associated antigen
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从模拟卵巢癌相关抗原的抗独特型抗体中鉴定出新的 T 细胞表位
DOI：
10.1007/s00262-007-0354-8
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发表时间：
2008-02
影响因子：
5.8
通讯作者：
Li, Wei
中科院分区：
文献类型：
--
作者：
Cui, Heng;Chang, Xiao-Hong;Li, Zi-Hai;Meng, Fan-Qiang;Zhang, Guo;Liu, Bei;Li, Wei
抗独特型（Id）抗体可诱导针对肿瘤抗原的特异性细胞免疫应答，但其抗原性机制尚不清楚。我们先前报道了一种抗Id抗体6 B11，其模拟人卵巢癌相关抗原OC 166 -9。为了探讨6 B11诱导细胞免疫反应的分子基础，合成了一组来自6 B11互补决定区（CDR）的肽。通过免疫学实验，我们发现6 B11的轻链和重链CDR 3分别为MHC Ⅰ类和Ⅱ类抗原表位。MHC I类和II类表位的组合比6 B11更有效地诱导针对卵巢癌的特异性细胞免疫应答。本研究为6 B11的抗原性提供了结构基础。6 B11中抗原特异性T细胞表位的鉴定为基于表位的卵巢癌疫苗的设计提供了依据。
Anti-idiotype (Id) antibodies can be used to induce specific cellular immune responses against tumor antigens, but the mechanism of antigenicity is not always clear. We previously reported an anti-Id antibody, 6B11, which mimics human ovarian cancer associated antigen OC166-9. To explore the molecular basis of cellular immune response induced by 6B11, a panel of peptides derived from complementarity determining region (CDR) of 6B11 were synthesized. After a series of immunologic experiments, we found that the light chain CDR3 peptide and heavy chain CDR3 peptide were the MHC class I and class II epitopes of 6B11, respectively. The combination of MHC class I and class II epitopes is more effective than 6B11 in inducing specific cellular immune response against ovarian cancer. Our study provided the structural basis of antigenicity of 6B11. The identification of antigen-specific T cell eptitopes in 6B11 should facilitate the design of epitope-based vaccine against human ovarian cancer.
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DOI：
--
发表时间：
1998-10
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
M. Pride;S. Shuey;A. Grillo‐López;G. Braslawsky;M. Ross;S. Legha;O. Eton;A. Buzaid;C. Ioannides;J. Murray
通讯作者：
M. Pride;S. Shuey;A. Grillo‐López;G. Braslawsky;M. Ross;S. Legha;O. Eton;A. Buzaid;C. Ioannides;J. Murray
影响因子：
4.4
作者：
Y. Dohi;K. Yamada;N. Ohno;M. Aoki;Y. Takagaki;A. Nisonoff;S. Shinka
通讯作者：
Y. Dohi;K. Yamada;N. Ohno;M. Aoki;Y. Takagaki;A. Nisonoff;S. Shinka
DOI：
--
发表时间：
1968
期刊：
Scandinavian journal of clinical and laboratory investigation. Supplementum
影响因子：
--
作者：
A. Böyum
通讯作者：
A. Böyum
影响因子：
4.4
作者：
T. Mosmann;H. Cherwinski;M. Bond;M. Giedlin;R. Coffman
通讯作者：
T. Mosmann;H. Cherwinski;M. Bond;M. Giedlin;R. Coffman
影响因子：
4.4
作者：
Rice, J;Elliott, T;Stevenson, FK
通讯作者：
Stevenson, FK