Abscisic acid promotes jasmonic acid biosynthesis via a 'SAPK10-bZIP72-AOC' pathway to synergistically inhibit seed germination in rice (Oryza sativa).

Abscisic acid promotes jasmonic acid biosynthesis via a 'SAPK10-bZIP72-AOC' pathway to synergistically inhibit seed germination in rice (Oryza sativa).
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脱落酸通过 SAPK10-bZIP72-AOC 途径促进茉莉酸生物合成,从而协同抑制水稻 (Oryza sativa) 种子发芽

DOI:
10.1111/nph.16774
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发表时间:
2020-11
期刊:
The New phytologist
影响因子:
--
通讯作者:
Zhang J
Zhang J
中科院分区:
其他
文献类型:
--
作者:
Wang Y;Hou Y;Qiu J;Wang H;Wang S;Tang L;Tong X;Zhang J

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脱落酸(ABA)和茉莉酸(JA)都抑制种子萌发,但它们在这一过程中的相互作用仍然难以捉摸。在这里,我们报告了一个‘SAPK10-bZIP72-AOC’途径,通过这个途径,ABA促进了茉莉酸的生物合成,从而协同抑制水稻种子的萌发。通过生化相互作用和磷酸化分析,我们发现SAPK10在第177位丝氨酸上表现出自动磷酸化活性,这使得它能够主要在第71位丝氨酸上磷酸化bZIP72。SAPK10依赖的磷酸化增强了bZIP72蛋白的稳定性以及与AOC启动子G-box顺式元件的DNA结合能力,从而提高了AOC转录和JA的内源浓度。抑制茉莉酸的合成显著降低了ABA对种子萌发的敏感性,表明ABA对种子萌发的抑制作用部分依赖于茉莉酸浓度的升高。我们的发现为水稻种子萌发过程中ABA-JA协同作用的分子网络提供了新的见解。
Abscisic acid (ABA) and jasmonic acid (JA) both inhibit seed germination, but their interactions during this process remain elusive. Here, we report the identification of a ‘SAPK10‐bZIP72‐AOC’ pathway, through which ABA promotes JA biosynthesis to synergistically inhibit rice seed germination. Using biochemical interaction and phosphorylation assays, we show that SAPK10 exhibits autophosphorylation activity on the 177th serine, which enables it to phosphorylate bZIP72 majorly on 71st serine. The SAPK10‐dependent phosphorylation enhances bZIP72 protein stability as well as the DNA‐binding ability to the G‐box cis‐element of AOC promoter, thereby elevating the AOC transcription and the endogenous concentration of JA. Blocking of JA biosynthesis significantly alleviated the ABA sensitivity on seed germination, suggesting that ABA‐imposed inhibition partially relied on the elevated concentration of JA. Our findings shed a novel insight into the molecular networks of ABA–JA synergistic interaction during rice seed germination.
SnRK2-APC/C(TE) 调节模块介导赤霉酸和脱落酸途径的拮抗作用。
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发表时间: 2014
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影响因子: 3.7
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