Localization of exercise- and denervation-responsive elements in the mouse GLUT4 gene.
Localization of exercise- and denervation-responsive elements in the mouse GLUT4 gene.
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小鼠 GLUT4 基因中运动和去神经反应元件的定位。
DOI：
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Osamu Ezaki
中科院分区：
文献类型：
--
作者：
Nobuyo Tsunoda;K. Maruyama;David W. Cooke;Daniel M. Lane;Osamu Ezaki
运动训练增加骨骼肌中GLUT 4的表达。先前的研究表明，小鼠GLUT 4基因的运动反应元件位于相对于转录起始位点的碱基-1001和-442之间。为了进一步表征GLUT 4基因中的调控元件，在转基因小鼠中研究了含有-701、-551、-442或-423 bp的5 '侧翼区的GLUT 4小基因的调控。研究的所有小基因在骨骼肌和心脏中均显示出显著表达，包括缺乏位于碱基-437和-428之间的肌细胞增强因子2（MEF 2）结合结构域（-CTAAAAAATAG-）的-423 GLUT 4小基因。-701-和-551-bp构建体在棕色脂肪组织中表达，而-442和-423构建体则不表达。在骨骼肌中，无论是游泳还是跑台都上调了-701和-551转基因小鼠的GLUT 4小基因mRNA水平，但在-442和-423转基因小鼠中没有。在所有-701、-551、-442和-423转基因小鼠中，通过切断坐骨神经下调小基因和内源性GLUT 4 mRNA对腓肠肌进行去神经支配。这些数据表明，运动反应元件和棕色脂肪细胞特异性元件位于GLUT 4基因的碱基-551和-442之间的109 bp内，但是去神经诱导的GLUT 4基因下调的顺式元件位于碱基-423的下游。最后，碱基-437和-428之间的MEF 2结合位点对于GLUT 4在骨骼肌和心脏中的表达不是必需的;介导这种作用的顺式元件也位于碱基-423的下游。
Exercise training increases the expression of GLUT4 in skeletal muscle. Previous studies demonstrated that the exercise-responsive element(s) of the murine GLUT4 gene are located between bases -1001 and -442 relative to the transcription start site. To further characterize the regulatory elements in the GLUT4 gene, the regulation of GLUT4 minigenes containing -701, -551, -442, or -423 bp of the 5'-flanking region was studied in transgenic mice. All minigenes studied showed significant expression in skeletal muscle and heart, including the -423 GLUT4 minigene that lacked the myocyte enhancer factor 2 (MEF2)-binding domain (-CTAAAAATAG-) located between bases -437 and -428. The -701- and -551-bp constructs were expressed in brown adipose tissues while the -442 and -423 constructs were not. In skeletal muscle, either swimming or treadmill running up-regulated GLUT4 minigene mRNA levels in -701 and -551 transgenic mice, but not in the -442 and -423 transgenic mice. Denervation of the gastrocnemius muscle by sectioning of the sciatic nerve down-regulated minigene and endogenous GLUT4 mRNAs in all -701, -551, -442, and -423 transgenic mice. These data indicate that exercise-responsive element(s) and brown adipocyte specific element(s) are located within 109 bp between bases -551 and -442 of the GLUT4 gene, but that the cis-element for denervation-induced down-regulation of the GLUT4 gene is located downstream of base -423. Finally, the MEF2 binding site between bases -437 and -428 is not necessary for expression of GLUT4 in skeletal muscles and heart; the cis-element mediating this effect is also located downstream of base -423.
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DOI：
10.1016/s0021-9258(19)36888-7
发表时间：
1993-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Jeanne M. Richardson;J. Pessin
通讯作者：
Jeanne M. Richardson;J. Pessin
大鼠 GLUT4/肌肉脂肪葡萄糖转运蛋白基因产物 5-异质性的表征。
DOI：
10.1210/endo.136.5.7720644
发表时间：
1995
期刊：
Endocrinology.
影响因子：
--
作者：
Olson,AL;Edgington,NP;Moye-Rowley,WS;Pessin,JE
通讯作者：
Pessin,JE
通过骨骼肌中 GLUT4 的转基因互补来预防 GLUT4 消除杂合子小鼠的胰岛素抵抗和糖尿病。
影响因子：
7.7
作者：
Tsao,TS;Stenbit,AE;Factor,SM;Chen,W;Rossetti,L;Charron,MJ
通讯作者：
Charron,MJ
影响因子：
18.2
作者：
J. Pessin;G. Bell
通讯作者：
J. Pessin;G. Bell
DOI：
--
发表时间：
1993-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Ren;B. Marshall;E. Gulve;Jiaping Gao;David W. Johnson;J. Holloszy;M. Mueckler
通讯作者：
J. Ren;B. Marshall;E. Gulve;Jiaping Gao;David W. Johnson;J. Holloszy;M. Mueckler