Serial mutational tracking in surgically resected locally advanced colorectal cancer with neoadjuvant chemotherapy.
Serial mutational tracking in surgically resected locally advanced colorectal cancer with neoadjuvant chemotherapy.
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DOI：
10.1038/s41416-018-0208-5
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发表时间：
2018-08
影响因子：
8.8
通讯作者：
Mimori K
中科院分区：
文献类型：
--
作者：
Sugimachi K;Sakimura S;Kuramitsu S;Hirata H;Niida A;Iguchi T;Eguchi H;Masuda T;Morita M;Toh Y;Maehara Y;Suzuki Y;Mimori K
我们的目的是研究连续基因突变追踪在新辅助化疗后接受根治性切除术的局部晚期结直肠癌患者中的应用。我们前瞻性收集了10例局部晚期结直肠癌患者，术前新辅助化疗后进行了根治性切除。在连续治疗期间获得来自原发性肿瘤、远处转移性肿瘤的组织和血浆。使用预先设计的基因面板和下一代测序对47个癌症相关基因进行综合突变分析。所有病例均显示对新辅助化疗的部分反应，并完成病理R0切除。在原发性肿瘤中，化疗前在1至14个位点检测到非同义突变，化疗后在1至2个位点检测到非同义突变。在纵向治疗期间，在血浆和转移性肿瘤中精确检测到创始人突变。系列突变分析表明，亚克隆选择发生在化疗过程中，血浆可以替代肿瘤组织的药物选择和治疗决策的突变分析。
We aim to investigate the utility of serial gene mutation tracking for locally advanced CRC in those who underwent curative resection following neoadjuvant chemotherapy. We prospectively collected 10 locally advanced CRC cases for which curative resection was performed following preoperative neoadjuvant chemotherapy. Tissues from the primary tumour, distant metastatic tumours, and blood plasma were obtained during serial treatment. Comprehensive mutation analysis of 47 cancer-associated genes was performed using a pre-designed gene panel and next-generation sequencing. All cases showed a partial response to neoadjuvant chemotherapy, and pathological R0 resection was accomplished. In primary tumours, non-synonymous mutations were detected at between 1 and 14 sites before chemotherapy and at between 1 and 2 sites after. Founder mutations were precisely detected in blood plasma and metastatic tumours during longitudinal treatment. Serial mutational analysis indicated that subclonal selection occurs during chemotherapy and that plasma can substitute for tumourous tissue in mutational analysis for drug selection and treatment decisions.
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影响因子：
8.4
作者：
Eisenhauer, E. A.;Therasse, P.;Verweij, J.
通讯作者：
Verweij, J.
通过新辅助化疗跟踪食管腺癌的基因组进化。
影响因子：
28.2
作者：
Murugaesu N;Wilson GA;Birkbak NJ;Watkins T;McGranahan N;Kumar S;Abbassi-Ghadi N;Salm M;Mitter R;Horswell S;Rowan A;Phillimore B;Biggs J;Begum S;Matthews N;Hochhauser D;Hanna GB;Swanton C
通讯作者：
Swanton C
影响因子：
2.3
作者：
Bhangu, Jagdeep Singh;Taghizadeh, Hossein;Mannhalter, Christine
通讯作者：
Mannhalter, Christine
DOI：
10.1073/pnas.0507904102
发表时间：
2005-11-08
影响因子：
11.1
作者：
Diehl, F;Li, M;Vogelstein, B
通讯作者：
Vogelstein, B
影响因子：
24.5
作者：
Barault L;Amatu A;Siravegna G;Ponzetti A;Moran S;Cassingena A;Mussolin B;Falcomatà C;Binder AM;Cristiano C;Oddo D;Guarrera S;Cancelliere C;Bustreo S;Bencardino K;Maden S;Vanzati A;Zavattari P;Matullo G;Truini M;Grady WM;Racca P;Michels KB;Siena S;Esteller M;Bardelli A;Sartore-Bianchi A;Di Nicolantonio F
通讯作者：
Di Nicolantonio F