Metabolomic profiling of Marek's disease virus infection in host cell based on untargeted LC-MS.
Metabolomic profiling of Marek's disease virus infection in host cell based on untargeted LC-MS.
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DOI：
10.3389/fmicb.2023.1270762
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发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
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作者：
马立克氏病（MD）是由马立克氏病病毒（MDV）引起的一种传染性疾病，可引起鸡的神经系统疾病和恶性淋巴瘤，严重威胁着养禽业的发展。然而，MDV如何破坏宿主细胞并引起损伤的潜在机制仍然是难以捉摸的。近年来，代谢组学在揭示病毒与宿主相互作用的复杂机制方面显示出巨大的潜力。采用超高效液相色谱-四极杆飞行时间串联质谱（UHPLC-QTOF-MS）技术，对MDV感染鸡胚成纤维细胞（CEFs）进行多因素分析。结果表明，MDV感染后，261种代谢产物发生了显著变化，其中以氨基酸代谢、能量代谢、核苷酸代谢和脂质代谢变化最多。值得注意的是，MDV感染在感染的早期阶段诱导宿主细胞中氨基酸的上调，以提供其自身复制的有效增殖所必需的能量和中间代谢物。总之，这些数据不仅在识别MDV在宿主细胞中复制所利用的生化分子方面有希望，而且还为理解MDV-宿主相互作用提供了新的见解。
Marek’s disease (MD) caused by Marek’s disease virus (MDV), poses a serious threat to the poultry industry by inducing neurological disease and malignant lymphoma in infected chickens. However, the underlying mechanisms how MDV disrupts host cells and causes damage still remain elusive. Recently, the application of metabolomics has shown great potential for uncovering the complex mechanisms during virus-host interactions. In this study, chicken embryo fibroblasts (CEFs) infected with MDV were subjected to ultrahigh-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS) and multivariate statistical analysis. The results showed that 261 metabolites were significantly altered upon MDV infection, with most changes occurring in amino acid metabolism, energy metabolism, nucleotide metabolism, and lipid metabolism. Notably, MDV infection induces an up-regulation of amino acids in host cells during the early stages of infection to provide the energy and intermediary metabolites necessary for efficient multiplication of its own replication. Taken together, these data not only hold promise in identifying the biochemical molecules utilized by MDV replication in host cells, but also provides a new insight into understanding MDV-host interactions.
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