Alterations in arthropod and neuronal exosomes reduce virus transmission and replication in recipient cells.
Alterations in arthropod and neuronal exosomes reduce virus transmission and replication in recipient cells.
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DOI：
10.20517/evcna.2022.30
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Sultana, Hameeda
中科院分区：
文献类型：
--
作者：
Fasae, Kehinde Damilare;Neelakanta, Girish;Sultana, Hameeda
针对病原体通过胞外体或胞外小泡的脱落/传播方式，已被认为是控制病媒传播疾病的最佳途径。这项研究的重点是改变外体的稳定性，以影响病原体从感染的受体细胞向幼稚的受体细胞的传播。在本研究中，神经元或节肢动物外体在不同温度、不同盐度或不同pH条件下处理，以分析它们从受感染的受体细胞向幼稚受体细胞传播森林病毒(LGTV)的能力和效率。实时定量聚合酶链式反应(qRT-PCR)和免疫印迹分析表明，分别在37℃或23℃的较高温度下，或用硫酸镁或硫酸铵等硫酸盐或高碱性pH为9或11.5的条件下处理神经元或壁虱外小体，可显著减少LGTV通过感染性外小体(人或壁虱细胞来源)向人类神经元(SH-SY5Y)细胞或皮肤角质形成细胞(HaCaT细胞)的传播。总体而言，这项研究表明，通过改变温度、盐或pH条件的基本变化来改变外切体的能力，可以减少外切体介导的病原体向脊椎动物宿主的病毒传播或病毒在哺乳动物宿主内或之间的传播和复制。
Targeting the modes of pathogen shedding/transmission via exosomes or extracellular vesicles has been envisioned as the best approach to control vector-borne diseases. This study is focused on altering exosomes stability to affect the pathogen transmission from infected to naïve recipient cells. In this study, neuronal or arthropod exosomes were treated at different temperatures or with different salts or pH conditions to analyze their ability and efficiency in the transmission of tick-borne Langat virus (LGTV) from infected to naïve recipient cells. Quantitative real-time PCR (qRT-PCR) and immunoblotting analyses revealed that treatment of neuronal or tick exosomes at warmer temperatures of 37 °C or 23 °C, respectively, or with sulfate salts such as Magnesium or Ammonium sulfates or with highly alkaline pH of 9 or 11.5, dramatically reduced transmission of LGTV via infectious exosomes (human or tick cells-derived) to human neuronal (SH-SY5Y) cells or skin keratinocytes (HaCaT cells), respectively. Overall, this study suggests that exosome-mediated viral transmission of vector-borne pathogens to the vertebrate host or the viral dissemination and replication within or between the mammalian host can be reduced by altering the ability of exosomes with basic changes in temperatures, salts or pH conditions.
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影响因子：
3.7
作者：
Macleod MK;David A;Jin N;Noges L;Wang J;Kappler JW;Marrack P
通讯作者：
Marrack P
DOI：
10.1186/s13690-021-00693-2
发表时间：
2021-10-19
期刊：
Archives of public health = Archives belges de sante publique
影响因子：
--
作者：
Hayashi T;Takeshita Y;Hutin YJ;Harmanci H;Easterbrook P;Hess S;van Holten J;Oru EO;Kaneko S;Yurdaydin C;Bulterys M
通讯作者：
Bulterys M
影响因子：
7.3
作者：
Ahmed W;Neelakanta G;Sultana H
通讯作者：
Sultana H
影响因子：
3.7
作者：
Johnston D;Earley B;McCabe MS;Kim J;Taylor JF;Lemon K;McMenamy M;Duffy C;Cosby SL;Waters SM
通讯作者：
Waters SM
影响因子：
7.5
作者：
Alem F;Olanrewaju AA;Omole S;Hobbs HE;Ahsan N;Matulis G;Brantner CA;Zhou W;Petricoin EF;Liotta LA;Caputi M;Bavari S;Wu Y;Kashanchi F;Hakami RM
通讯作者：
Hakami RM