The bacterial composition within the Sarracenia purpurea model system: local scale differences and the relationship with the other members of the food web.
The bacterial composition within the Sarracenia purpurea model system: local scale differences and the relationship with the other members of the food web.
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DOI：
10.1371/journal.pone.0050969
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Kostka JE
中科院分区：
文献类型：
--
作者：
Gray SM;Akob DM;Green SJ;Kostka JE
肉食性猪笼草的叶子含有一个微小的水生食物网，被认为是生态学研究中的一个模型系统。这一食物链的中间和最高营养级的物种身份，以及有害的更多的吸浆虫，在紫斑潜蝇原生地理范围内高度相似，在某些情况下，似乎与植物共同进化。然而，直到最近，这个食物网底层营养水平的细菌的身份、地理变异和多样性在很大程度上仍不为人所知。本研究调查了紫云英叶片内的细菌群落组成，以解决：1)在植物原生地理范围的不同区域(南部和中部)，在局部尺度上演替开始时细菌群落的变化；2)细菌消费者和水生食物网的其他成员(即昆虫)对细菌群落结构的影响。用16S核糖体RNA基因克隆技术分析了来自纽约和佛罗里达研究地点的六片叶子(每株一片)的群落。每个地点内的每个水罐都有一个不同的群落；然而，与跨地点比较群落时相比，每个地点内的细菌组成有更多的重叠。相反，该群落中原生动物和后生动物的物种同一性在同一地点内和两个地点之间都是相似的，但丰度不同。我们的结果表明，至少在演替的开始，没有对细菌分类群的强烈选择，也没有植物开始分解被困昆虫所需的核心细菌群。植物和细菌之间的共同进化似乎没有像这个群落的其他成员那样发生。
The leaves of the carnivorous pitcher plant, Sarracenia purpurea, contain a microscopic aquatic food web that is considered a model system in ecological research. The species identity of the intermediate and top trophic level of this food web, as well the detritivore midge, are highly similar across the native geographic range of S. purpurea and, in some cases, appear to have co-evolved with the plant. However, until recently, the identity, geographic variation, and diversity of the bacteria in the bottom trophic level of this food web have remained largely unknown. This study investigated bacterial community composition inside the leaves of S. purpurea to address: 1) variation in bacterial communities at the beginning of succession at the local scale in different areas of the plant’s native geographic range (southern and mid-regional sites) and 2) the impacts of bacterial consumers and other members of the aquatic food web (i.e., insects) on bacterial community structure. Communities from six leaves (one leaf per plant) from New York and Florida study sites were analyzed using 16S ribosomal RNA gene cloning. Each pitcher within each site had a distinct community; however, there was more overlap in bacterial composition within each site than when communities were compared across sites. In contrast, the identity of protozoans and metazoans in this community were similar in species identity both within a site and between the two sites, but abundances differed. Our results indicate that, at least during the beginning of succession, there is no strong selection for bacterial taxa and that there is no core group of bacteria required by the plant to start the decomposition of trapped insects. Co-evolution between the plant and bacteria appears to not have occurred as it has for other members of this community.
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影响因子：
14.9
作者：
Ludwig, W;Strunk, O;Schleifer, KH
通讯作者：
Schleifer, KH
影响因子：
2.6
作者：
Gray, Sarah M.;Miller, Thomas E.;Daufresne, Tanguy
通讯作者：
Daufresne, Tanguy
影响因子：
4.2
作者：
Akob, Denise M.;Mills, Heath J.;Kostka, Joel E.
通讯作者：
Kostka, Joel E.
影响因子：
6.4
作者：
Buckley, Hannah L.;Miller, Thomas E.;Gotelli, Nicholas J.
通讯作者：
Gotelli, Nicholas J.
影响因子：
1
作者：
Godt, MJW;Hamrick, JL
通讯作者：
Hamrick, JL