Expression of AEG-1 mRNA and protein in colorectal cancer patients and colon cancer cell lines.
Expression of AEG-1 mRNA and protein in colorectal cancer patients and colon cancer cell lines.
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DOI：
10.1186/1479-5876-10-109
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发表时间：
2012-05-29
影响因子：
7.4
通讯作者：
Sun XF
中科院分区：
文献类型：
--
作者：
Gnosa S;Shen YM;Wang CJ;Zhang H;Stratmann J;Arbman G;Sun XF
星形胶质细胞升高基因1（AEG-1）是一种重要的癌基因，已被证明在几种类型的癌症中过表达。在结直肠癌（CRC）中，与正常粘膜相比，AEG-1的蛋白水平在肿瘤组织中上调，显示出预后意义。由于AEG-1在大肠癌中的表达水平及其生物学途径尚不清楚，本研究旨在探讨AEG-1 mRNA表达水平、蛋白水平与大肠癌临床病理变量的关系及其生物学途径。通过qPCR分析了新鲜冷冻患者样品中AEG-1的mRNA表达，所述新鲜冷冻患者样品包括156个原发性肿瘤，沿着相应的正常粘膜，以及5种结肠癌细胞系SW 480、SW 620、KM 12 C、KM 12 SM和KM 12 L4 a。应用免疫组化方法检测74例远端正常黏膜、107例癌旁黏膜、158例原发性肿瘤、35例淋巴结转移和9例肝转移石蜡包埋标本中AEG-1蛋白的表达。此外，AEG-1蛋白的表达在细胞系中通过Western blot来阐明。淋巴结转移细胞系SW 620的AEG-1 mRNA表达（0.27 ± 0.02）显著高于原发性肿瘤细胞系SW 480（0.17 ± 0.04，p = 0.026）。AEG-1在mRNA和/或蛋白水平的表达从正常黏膜到原发灶、淋巴结转移灶、肝转移灶均呈明显上调（p < 0.05）。AEG-1 mRNA表达与肿瘤位置显著相关（p = 0.047），AEG-1 mRNA和蛋白表达与肿瘤分期显著相关（p < 0.03）。AEG-1蛋白表达与NF-κB、p73、Rad 50、凋亡等生物学指标呈正相关（p < 0.05）。AEG-1在mRNA和蛋白质水平上在CRC发展和侵袭过程中上调，并且与肿瘤位置和阶段相关。可能通过NF-κB信号通路在结直肠癌中发挥作用。
Astrocyte elevated gene 1 (AEG-1), an important oncogene, has been shown to be overexpressed in several types of cancers. In colorectal cancer (CRC), the protein level of AEG-1 is up-regulated in tumour tissue compared to normal mucosa, showing prognostic significance. Since little is known about the transcriptional level of AEG-1 expression and its biological pathway in CRC the aim of the present study was to examine the relationship of AEG-1 mRNA expression, the protein level and clinicopathological variables as well as its biology pathway in CRC. The mRNA expression of AEG-1 was analysed by qPCR in fresh frozen patient samples including 156 primary tumours, along with the corresponding normal mucosa, and in five colon cancer cell lines, SW480, SW620, KM12C, KM12SM and KM12L4a. AEG-1 protein expression was investigated by immunohistochemistry in paraffin-embedded materials from 74 distant normal mucosa, 107 adjacent mucosa, 158 primary tumour, 35 lymph node metastasis and 9 liver metastasis samples. In addition, the AEG-1 protein expression was elucidated in the cell lines by Western blot. The lymph node metastatic cell line SW620 had a significantly higher AEG-1 mRNA (0.27 ± 0.02) expression compared to the primary tumour cell line SW480 (0.17 ± 0.04, p = 0.026). AEG-1 expression at the mRNA level and/or the protein level was significantly up-regulated gradually from normal mucosa to primary CRC, and then to lymph node metastasis and finally to liver metastasis (p < 0.05). There were significant associations of AEG-1 mRNA expression with tumour location (p = 0.047), as well as mRNA and protein expression with the tumour stage (p < 0.03). Furthermore AEG-1 protein expression was positively related to biological variables including NF-κB, p73, Rad50 and apoptosis (p < 0.05). AEG-1 is up-regulated, at the mRNA and the protein level, during CRC development and aggressiveness, and is related to tumour location and stage. It may play its role in CRC through the NF-κB signaling pathway.
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影响因子：
50.3
作者：
Hu G;Chong RA;Yang Q;Wei Y;Blanco MA;Li F;Reiss M;Au JL;Haffty BG;Kang Y
通讯作者：
Kang Y
影响因子：
7.3
作者：
Kapiteijn, E;Liefers, GJ;van Krieken, JHJM
通讯作者：
van Krieken, JHJM
影响因子：
--
作者：
Gao, Jingfang;Zhang, Hong;Sun, Xiao-Feng
通讯作者：
Sun, Xiao-Feng
影响因子：
5.2
作者：
Martin, Angel G.;Trama, Jason;Fearnhead, Howard O.
通讯作者：
Fearnhead, Howard O.
肾细胞癌中 AEG-1 的过度表达及其与肿瘤核分级和进展的相关性。
影响因子：
3
作者：
Chen, W.;Ke, Z.;Wang, L.
通讯作者：
Wang, L.