Urinary CD133+ extracellular vesicles are decreased in kidney transplanted patients with slow graft function and vascular damage.
Urinary CD133+ extracellular vesicles are decreased in kidney transplanted patients with slow graft function and vascular damage.
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DOI：
10.1371/journal.pone.0104490
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Bussolati B
中科院分区：
文献类型：
--
作者：
Dimuccio V;Ranghino A;Praticò Barbato L;Fop F;Biancone L;Camussi G;Bussolati B
尿液中存在的细胞外囊泡（EV）主要由肾单位细胞释放，因此可以提供有关肾功能的信息。我们在此评估了正常受试者和肾移植患者尿液中表达祖细胞标志物CD133的囊泡的存在。我们发现，EV表达CD 133存在于正常人的尿液中，而不是终末期肾病患者。移植后第一天，尿CD133+ EV以低水平存在，此后（第7天）增加。与早期移植肾功能恢复的患者相比，移植肾功能恢复缓慢的患者尿CD133+ EV显著增加。在移植前移植物严重血管损伤的患者中，CD133+ EV在第7天没有增加。在方差分析中，与对照组相比，终末期肾病患者或移植患者尿液中表达肾外泌体标志物CD24的EV水平没有变化。发现分选的CD 133 + EV表达肾小球和近端肾小管标记物。这些数据表明，尿CD133+ EV在肾单位的稳态周转期间持续释放，并可提供有关其功能或再生潜力的信息。
Extracellular vesicles (EVs) present in the urine are mainly released from cells of the nephron and can therefore provide information on kidney function. We here evaluated the presence of vesicles expressing the progenitor marker CD133 in the urine of normal subjects and of patients undergoing renal transplant. We found that EV expressing CD133 were present in the urine of normal subjects, but not of patients with end stage renal disease. The first day after transplant, urinary CD133+ EVs were present at low levels, to increase thereafter (at day 7). Urinary CD133+ EVs significantly increased in patients with slow graft function in respect to those with early graft function. In patients with a severe pre-transplant vascular damage of the graft, CD133+ EVs did not increase at day 7. At variance, the levels of EVs expressing the renal exosomal marker CD24 did not vary in the urine of patients with end stage renal disease or in transplanted patients in respect to controls. Sorted CD133+ EVs were found to express glomerular and proximal tubular markers. These data indicate that urinary CD133+ EVs are continuously released during the homeostatic turnover of the nephron and may provide information on its function or regenerative potential.
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影响因子：
11.1
作者：
Bauer, Nicola;Wilsch-Braeuninger, Michaela;Karbanova, Jana;Fonseca, Ana-Violeta;Strauss, Doreen;Freund, Daniel;Thiele, Christoph;Huttner, Wieland B.;Bornhaeuser, Martin;Corbeil, Denis
通讯作者：
Corbeil, Denis
影响因子：
7.3
作者：
Smeets, Bart;Boor, Peter;Dijkman, Henry;Sharma, Shagun V.;Jirak, Peggy;Mooren, Fieke;Berger, Katja;Bornemann, Joerg;Gelman, Irwin H.;Floege, Juergen;van der Vlag, Johan;Wetzels, Jack F. M.;Moeller, Marcus J.
通讯作者：
Moeller, Marcus J.
DOI：
10.1152/ajprenal.00184.2011
发表时间：
2012-01-01
影响因子：
4.2
作者：
Bussolati, Benedetta;Moggio, Aldo;Camussi, Giovanni
通讯作者：
Camussi, Giovanni
影响因子：
19.6
作者：
Keller, S.;Rupp, C.;Altevogt, P.
通讯作者：
Altevogt, P.
影响因子：
13.6
作者：
Hogan, Marie C.;Manganelli, Luca;Ward, Christopher J.
通讯作者：
Ward, Christopher J.