Haematopoietic stem cell differentiation promotes the release of prominin-1/CD133-containing membrane vesicles--a role of the endocytic-exocytic pathway.
Haematopoietic stem cell differentiation promotes the release of prominin-1/CD133-containing membrane vesicles--a role of the endocytic-exocytic pathway.
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DOI：
10.1002/emmm.201100147
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发表时间：
2011-07
影响因子：
11.1
通讯作者：
Corbeil, Denis
中科院分区：
文献类型：
--
作者：
Bauer, Nicola;Wilsch-Braeuninger, Michaela;Karbanova, Jana;Fonseca, Ana-Violeta;Strauss, Doreen;Freund, Daniel;Thiele, Christoph;Huttner, Wieland B.;Bornhaeuser, Martin;Corbeil, Denis
干细胞的分化是细胞生物学中的一个基本过程，了解其机制可能会为治疗策略开辟新的途径。使用体外共培养系统的人原代造血干细胞和祖细胞生长的多能间充质基质细胞作为饲养细胞层，我们在这里描述的外泌体介导的释放的小膜囊泡含有干细胞和癌症干细胞标志物CD 133 -1（CD 133）在造血细胞分化。令人惊讶的是，这与神经祖细胞质膜突起释放胆固醇结合蛋白的出芽机制形成对比。然而，在这两种祖细胞类型，蛋白质-脂质组件可能是必不可少的结构决定因素，在释放过程中的repeatin-1。总的来说，这些数据支持这样的概念，即含β-内酰胺酶-1的脂筏可能是维持干细胞特性所必需的关键决定因素，它们的定量减少或丢失可能导致细胞分化。
The differentiation of stem cells is a fundamental process in cell biology and understanding its mechanism might open a new avenue for therapeutic strategies. Using an ex vivo co-culture system consisting of human primary haematopoietic stem and progenitor cells growing on multipotent mesenchymal stromal cells as a feeder cell layer, we describe here the exosome-mediated release of small membrane vesicles containing the stem and cancer stem cell marker prominin-1 (CD133) during haematopoietic cell differentiation. Surprisingly, this contrasts with the budding mechanism underlying the release of this cholesterol-binding protein from plasma membrane protrusions of neural progenitors. Nevertheless, in both progenitor cell types, protein–lipid assemblies might be the essential structural determinant in the release process of prominin-1. Collectively, these data support the concept that prominin-1-containing lipid rafts may host key determinants necessary to maintain stem cell properties and their quantitative reduction or loss may result in cellular differentiation.
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DOI：
10.1006/bbrc.2001.5271
发表时间：
2001-07-27
影响因子：
3.1
作者：
Corbeil, D;Fargeas, CA;Huttner, WB
通讯作者：
Huttner, WB
影响因子：
11.2
作者：
Vander Griend DJ;Karthaus WL;Dalrymple S;Meeker A;DeMarzo AM;Isaacs JT
通讯作者：
Isaacs JT
影响因子：
4
作者：
Freund, Daniel;Bauer, Nicola;Corbeil, Denis
通讯作者：
Corbeil, Denis
影响因子：
4.8
作者：
Bakhti, Mostafa;Winter, Christine;Simons, Mikael
通讯作者：
Simons, Mikael
鉴定非典型畸胎组织/色肽肿瘤中CD133阳性辐射细胞。
影响因子：
3.7
作者：
Chiou, Shih-Hwa;Kao, Chung-Lan;Chen, Yi-Wei;Chien, Chien-Shu;Hung, Shih-Chieh;Lo, Jeng-Fan;Chen, Yann-Jang;Ku, Hung-Hai;Hsu, Ming-Ta;Wong, Tai-Tong
通讯作者：
Wong, Tai-Tong