Local relaxin biosynthesis in the ovary and uterus through the oestrous cycle and early pregnancy in the female marmoset monkey (Callithrix jacchus).
Local relaxin biosynthesis in the ovary and uterus through the oestrous cycle and early pregnancy in the female marmoset monkey (Callithrix jacchus).
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雌性狨猴 (Callithrix jacchus) 发情周期和妊娠早期卵巢和子宫的局部松弛素生物合成。
DOI：
10.1093/humrep/12.6.1325
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发表时间：
1997
影响因子：
6.1
通讯作者：
R. Ivell
中科院分区：
文献类型：
--
作者：
A. Einspanier;M. R. Zarreh;M. Balvers;L. Kerr;K. Fuhrmann;R. Ivell
女性外周血中多肽激素松弛素的浓度模式表明，在月经周期和早期怀孕期间，可能存在有趣的旁分泌功能。为了详细研究这一生理学，决定在已建立的人类雌性生殖功能的灵长类动物模型-绒猴(Callithrix Jacchus)中检测局部松弛素的生物合成。在这项最初的研究中，使用分子和免疫学技术相结合的方法，在绒猴的发情周期中评估了松弛素的生物合成。从绒猴卵巢中克隆了松弛蛋白基因的全长转录本的核苷酸序列，发现其与人H2松弛蛋白的核苷酸序列具有较高的同源性。利用从该序列获得的基因特异性探针，通过核糖核酸酶保护试验、逆转录聚合酶链式反应(RT-PCR)和原位杂交，发现松弛蛋白基因在发情周期的卵泡膜细胞和黄体细胞中的表达，在妊娠早期增加。松弛素基因在雄性绒猴的子宫和胎盘中的表达水平较低，在前列腺中的表达水平较高。用两种不同的松弛素特异性抗血清，在卵巢内观察到松弛素样免疫反应，其分布规律与原位杂交结果一致。在非妊娠子宫中，在增殖期晚期的子宫内膜上皮中也发现了免疫反应，在分泌期的腺体中免疫反应增强。综上所述，松弛素肽和mRNA的表达模式表明，在这个新世界的猴子发情周期中，卵巢卵泡和黄体内以及子宫内存在局部松弛素生理基础。
The pattern of peripheral serum concentration for the peptide hormone relaxin in women points to the possibility of an interesting paracrine function in the cycle and early pregnancy. In order to investigate this physiology in detail, it was decided to examine local relaxin biosynthesis in an established primate model for human female reproductive function, the marmoset monkey (Callithrix jacchus). In this initial study relaxin biosynthesis was assessed using a combination of molecular and immunological techniques through the oestrous cycle in the marmoset monkey. The nucleotide sequence of the full-length relaxin gene transcript was cloned from the marmoset ovary and found to be closely homologous to that of the human H2 relaxin. Using gene specific probes derived from this sequence, RNase protection assays, reverse transcription-polymerase chain reaction (RT-PCR) assays and in-situ hybridization, showed relaxin gene expression within the ovary in theca cells and corpora lutea in the oestrous cycle, increasing in early pregnancy. Relaxin gene expression was also identified at a low level in the uterus and placenta, and at a higher level in the prostate in the male marmoset monkey. Using two different relaxin-specific antisera, relaxin-like immunoreactivity was observed in the ovary with a pattern of distribution coincident with that obtained by in-situ hybridization. Immunoreactivity was also found in the non-pregnant uterus, within the endometrial epithelium of the late proliferative phase and increasing within the glands through the secretory phase. Taken together, the pattern of relaxin peptide and mRNA expression show there is the basis for local relaxin physiology within the ovarian follicle and corpus luteum, and within the uterus during the oestrous cycle in this new world monkey.
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DOI：
10.1210/jcem-71-4-889
发表时间：
1990
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
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作者：
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Tseng,L
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影响因子：
3.6
作者：
Stoelk,E;Chegini,N;Lei,ZM;Rao,CV;Bryant-Greenwood,G;Sanfilippo,J
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影响因子：
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作者：
Gagliardi,CL;Goldsmith,LT;Saketos,M;Weiss,G;Schmidt,CL
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影响因子：
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作者：
Zhang,Q;Bagnell,CA
通讯作者：
Bagnell,CA
催产素和松弛素对大鼠子宫段收缩力的拮抗作用。
影响因子：
9.8
作者：
Goldsmith,LT;Skurnick,JH;Wojtczuk,AS;Linden,M;Kuhar,MJ;Weiss,G
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Weiss,G