Polymeric microsphere enhanced surface plasmon resonance imaging immunosensor for occult blood monitoring
Polymeric microsphere enhanced surface plasmon resonance imaging immunosensor for occult blood monitoring
复制标题
用于潜血监测的聚合物微球增强表面等离子共振成像免疫传感器
DOI：
10.1016/j.snb.2021.130858
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发表时间：
2022-01
期刊：
影响因子：
--
通讯作者：
Yonghong Shao
中科院分区：
文献类型：
--
作者：
Jie Zhou;Xueliang Wang;Jiajie Chen;Youjun Zeng;Dayong Gu;Bruce Zhi Gao;Yonghong Shao
潜血试验是一种常规筛查方法，用于评估泌尿系统和消化道的功能。人体排泄物中的血红蛋白（Hb）含量是存在潜血的重要指标。在这项研究中，表面等离子体共振成像（SPRi）为基础的免疫传感器具有高灵敏度的检测血红蛋白。通过将抗Hb抗体物理固定在检测通道上构建免疫传感器。将用于评价的样品与聚合物微球-抗体缀合物预孵育。真实的实时监测微球-抗体-抗原和固定化抗体的特异性结合引起的共振波长的偏移。由于微球的放大效应，在固定化抗体浓度为10 μg/mL、微球-抗体偶联物稀释比为1：200、微球-抗体偶联物与样品孵育时间为10 min的优化条件下，免疫传感器的检测限降低了42倍，为4.8 ng/mL。该免疫传感器在10 ng/mL ~ 500 ng/mL范围内对血红蛋白有良好的线性响应，对血红蛋白有良好的特异性，对尿样中血红蛋白的回收率可接受。并对50份临床尿样进行了检测。因此，本研究所开发的SPRi免疫传感器为潜血的检测提供了一种简单、快速、高通量和灵敏的免疫学策略。
Occult blood testing is a routine screening method for evaluating the function of the urinary system and digestive tract. The hemoglobin (Hb) content in human excreta is an important indicator of the presence of occult blood. In this study, a surface plasmon resonance imaging (SPRi)-based immunosensor with high sensitivity was developed for the detection of Hb. The immunosensor was constructed by physically immobilizing anti-Hb antibodies on the detection channels. Samples for evaluation were pre-incubated with the polymeric microsphere-antibody conjugate. The shift in resonance wavelength induced by the specific binding of microsphere-antibody-antigen and immobilized antibodies was monitored in real time. Owing to the amplification effect of microspheres, the detection limit of the immunosensor was reduced 42-fold to 4.8 ng/mL under the optimized concentration of immobilized antibody (10 μg/mL) with microsphere-antibody conjugate dilution ratio of 1:200 and incubation time of the microsphere-antibody conjugate and samples (10 min). The immunosensor displayed a good linear response to Hb ranging from 10 ng/mL to 500 ng/mL in both test solutions and in 1:30 urine sample, as well as a good specificity toward Hb and an acceptable recovery rate for spiked Hb solution in urine sample. The immunosensor was also exploited to detect the Hb concentration in 50 clinical urine samples. Therefore, the proposed SPRi immunosensor developed in this study provides a simple, rapid, high-throughput, and sensitive immunology strategy for the detection of occult blood.
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DOI：
10.1086/375896
发表时间：
2003-08
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
C. Gill;J. Lau;S. Gorbach;D. Hamer
通讯作者：
C. Gill;J. Lau;S. Gorbach;D. Hamer
影响因子：
3.8
作者：
Lee, Sang Hun;Sung, Jong Hwan;Park, Tai Hyun
通讯作者：
Park, Tai Hyun
影响因子：
4.8
作者：
N. Swaminathan;Yowan Nerthigan;Hui-Fen Wu
通讯作者：
N. Swaminathan;Yowan Nerthigan;Hui-Fen Wu
DOI：
10.3760/cma.j.issn.1009-9158.2017.04.016
发表时间：
2017-04
影响因子：
--
作者：
Ping Wang;Hui Liu;Tie Zhang
通讯作者：
Ping Wang;Hui Liu;Tie Zhang
影响因子：
3.7
作者：
Garcés-Chávez, V;Quidant, R;Dholakia, K
通讯作者：
Dholakia, K