An observational study of anaerobic bacteria in cystic fibrosis lung using culture dependant and independent approaches.
An observational study of anaerobic bacteria in cystic fibrosis lung using culture dependant and independent approaches.
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DOI：
10.1038/s41598-021-85592-w
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发表时间：
2021-03-25
影响因子：
4.6
通讯作者：
Héry-Arnaud G
中科院分区：
文献类型：
--
作者：
Lamoureux C;Guilloux CA;Beauruelle C;Gouriou S;Ramel S;Dirou A;Le Bihan J;Revert K;Ropars T;Lagrafeuille R;Vallet S;Le Berre R;Nowak E;Héry-Arnaud G
严格厌氧菌是囊性纤维化（CF）肺的重要居民，但仍然是未知的。本研究的主要目的是描述CF气道微生物群中厌氧菌的多样性，并评估其与肺功能的相关性。在8个月内进行了一项观察性研究。101例患者入组本研究，并使用设计用于保持厌氧条件的无菌样本套件采集150份痰液样本。开发了112个细菌的扩大培养方法和141个细菌的分子方法（针对CF肺中的三个主要厌氧属：普雷沃氏菌、韦荣氏菌和梭杆菌的定量PCR）。在培养中，91.1%的嗜酸菌至少对一种厌氧菌呈阳性，平均每痰检出6种厌氧菌。共检出厌氧菌31属69种，是迄今为止报道的CF肺厌氧菌多样性最高的。较好的肺功能（定义为一秒用力呼气容积> 70%）与较高的韦荣氏球菌定量显著相关。这些结果提出了厌氧菌对肺功能的潜在影响的问题。
Strict anaerobes are undeniably important residents of the cystic fibrosis (CF) lung but are still unknowns. The main objectives of this study were to describe anaerobic bacteria diversity in CF airway microbiota and to evaluate the association with lung function. An observational study was conducted during eight months. A hundred and one patients were enrolled in the study, and 150 sputum samples were collected using a sterile sample kit designed to preserve anaerobic conditions. An extended-culture approach on 112 sputa and a molecular approach (quantitative PCR targeting three of the main anaerobic genera in CF lung: Prevotella, Veillonella, and Fusobacterium) on 141 sputa were developed. On culture, 91.1% of sputa were positive for at least one anaerobic bacterial species, with an average of six anaerobic species detected per sputum. Thirty-one anaerobic genera and 69 species were found, which is the largest anaerobe diversity ever reported in CF lungs. Better lung function (defined as Forced Expiratory Volume in one second > 70%) was significantly associated with higher quantification of Veillonella. These results raise the question of the potential impact of anaerobes on lung function.
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在囊性纤维化中，跨年龄和疾病阶段的肺微生物群。
影响因子：
4.6
作者：
Coburn B;Wang PW;Diaz Caballero J;Clark ST;Brahma V;Donaldson S;Zhang Y;Surendra A;Gong Y;Elizabeth Tullis D;Yau YC;Waters VJ;Hwang DM;Guttman DS
通讯作者：
Guttman DS
DOI：
10.1164/rccm.201505-0943oc
发表时间：
2015-12-01
影响因子：
24.7
作者：
Mirkovic, Bojana;Murray, Michelle A.;McElvaney, Noel G.
通讯作者：
McElvaney, Noel G.
影响因子：
24.3
作者：
Muhlebach, Marianne S.;Hatch, Joseph E.;Tunney, Michael M.
通讯作者：
Tunney, Michael M.
影响因子：
8.3
作者：
Chmiel, James F;Aksamit, Timothy R;Ratjen, Felix A
通讯作者：
Ratjen, Felix A
影响因子：
6.4
作者：
Cowley ES;Kopf SH;LaRiviere A;Ziebis W;Newman DK
通讯作者：
Newman DK