Deeper penetration of erythrocytes into the endothelial glycocalyx is associated with impaired microvascular perfusion.
Deeper penetration of erythrocytes into the endothelial glycocalyx is associated with impaired microvascular perfusion.
复制标题
DOI：
10.1371/journal.pone.0096477
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
NEO study group
中科院分区：
文献类型：
--
作者：
Lee DH;Dane MJ;van den Berg BM;Boels MG;van Teeffelen JW;de Mutsert R;den Heijer M;Rosendaal FR;van der Vlag J;van Zonneveld AJ;Vink H;Rabelink TJ;NEO study group
内皮糖萼的变化是心血管疾病发展中最早的变化之一。内皮糖萼既是流动血液与血管壁之间相互作用的重要生物调节剂，也是器官灌注的决定因素。我们假设红细胞更深地渗透到糖萼中与微血管灌注减少有关。这项基于人群的前瞻性队列研究（荷兰肥胖流行病学 [NEO] 研究）包括 6,673 名中年个体（对超重和肥胖个体进行过采样）。在该队列中，我们使用侧流暗场 (SDF) 成像以及最近开发的自动采集和分析方法对 915 名参与者的舌下微脉管系统进行了成像。在红细胞柱宽 5 至 25 µm 的血管中评估红细胞（作为微血管灌注的标志）和灌注边界区域 (PBR) 的存在，PBR 是红细胞可及性的内皮糖萼屏障特性的标志。观察到 PBR 测量值存在很大的差异，平均 PBR 为 2.14 µm（范围：1.43–2.86 µm）。线性回归分析显示，PBR 与微血管灌注之间存在显着相关性，这通过红细胞充盈百分比反映出来（回归系数 β：-0.034；95% 置信区间：-0.037 至 -0.031）。我们得出的结论是，具有厚（“健康”）糖萼（低 PBR）的微血管床反映了微血管床的有效灌注。相反，薄的（“风险”）糖萼（高 PBR）与微血管灌注效率较低和有缺陷相关。
Changes in endothelial glycocalyx are one of the earliest changes in development of cardiovascular disease. The endothelial glycocalyx is both an important biological modifier of interactions between flowing blood and the vessel wall, and a determinant of organ perfusion. We hypothesize that deeper penetration of erythrocytes into the glycocalyx is associated with reduced microvascular perfusion. The population-based prospective cohort study (the Netherlands Epidemiology of Obesity [NEO] study) includes 6,673 middle-aged individuals (oversampling of overweight and obese individuals). Within this cohort, we have imaged the sublingual microvasculature of 915 participants using sidestream darkfield (SDF) imaging together with a recently developed automated acquisition and analysis approach. Presence of RBC (as a marker of microvascular perfusion) and perfused boundary region (PBR), a marker for endothelial glycocalyx barrier properties for RBC accessibility, were assessed in vessels between 5 and 25 µm RBC column width. A wide range of variability in PBR measurements, with a mean PBR of 2.14 µm (range: 1.43–2.86 µm), was observed. Linear regression analysis showed a marked association between PBR and microvascular perfusion, reflected by RBC filling percentage (regression coefficient β: −0.034; 95% confidence interval: −0.037 to −0.031). We conclude that microvascular beds with a thick (“healthy”) glycocalyx (low PBR), reflects efficient perfusion of the microvascular bed. In contrast, a thin (“risk”) glycocalyx (high PBR) is associated with a less efficient and defective microvascular perfusion.
登录
查看更多内容
影响因子：
3.8
作者：
Goedhart, P. T.;Khalilzada, M.;Ince, C.
通讯作者：
Ince, C.
影响因子：
5.7
作者：
Klijn, Eva;Den Uil, C. A.;Ince, Can
通讯作者：
Ince, Can
影响因子：
--
作者：
DESJARDINS, C;DULING, BR
通讯作者：
DULING, BR
DOI：
10.2215/cjn.08160813
发表时间：
2014-04-07
影响因子：
9.8
作者：
Dane, Martijn J. C.;Khairoun, Meriem;Rabelink, Ton J.
通讯作者：
Rabelink, Ton J.
影响因子：
3.3
作者：
Nieuwdorp, Max;Meuwese, Marijn C.;Vink, Hans
通讯作者：
Vink, Hans