The Vfl1 Protein in Chlamydomonas localizes in a rotationally asymmetric pattern at the distal ends of the basal bodies.
The Vfl1 Protein in Chlamydomonas localizes in a rotationally asymmetric pattern at the distal ends of the basal bodies.
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DOI：
10.1083/jcb.153.1.63
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发表时间：
2001-04-02
期刊：
影响因子：
--
通讯作者：
Lefebvre PA
中科院分区：
文献类型：
--
作者：
Silflow CD;LaVoie M;Tam LW;Tousey S;Sanders M;Wu W;Borodovsky M;Lefebvre PA
In the unicellular alga Chlamydomonas, two anterior flagella are positioned with 180° rotational symmetry, such that the flagella beat with the effective strokes in opposite directions (Hoops, H.J., and G.B. Witman. 1983. J. Cell Biol. 97:902–908). The vfl1 mutation results in variable numbers and positioning of flagella and basal bodies (Adams, G.M.W., R.L. Wright, and J.W. Jarvik. 1985. J. Cell Biol. 100:955–964). Using a tagged allele, we cloned the VFL1 gene that encodes a protein of 128 kD with five leucine-rich repeat sequences near the NH2 terminus and a large α-helical–coiled coil domain at the COOH terminus. An epitope-tagged gene construct rescued the mutant phenotype and expressed a tagged protein (Vfl1p) that copurified with basal body flagellar apparatuses. Immunofluorescence experiments showed that Vfl1p localized with basal bodies and probasal bodies. Immunogold labeling localized Vfl1p inside the lumen of the basal body at the distal end. Distribution of gold particles was rotationally asymmetric, with most particles located near the doublet microtubules that face the opposite basal body. The mutant phenotype, together with the localization results, suggest that Vfl1p plays a role in establishing the correct rotational orientation of basal bodies. Vfl1p is the first reported molecular marker of the rotational asymmetry inherent to basal bodies.
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影响因子：
7.8
作者：
ADAMS, GMW;WRIGHT, RL;JARVIK, JW
通讯作者：
JARVIK, JW
基础与身体相关的Ca2+结合蛋白的纯化和表征。
影响因子：
7.8
作者：
Huang, B;Watterson, D M;Lee, V D;Schibler, M J
通讯作者：
Schibler, M J
DOI：
10.1083/jcb.40.3.716
发表时间：
1969-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Allen RD
通讯作者：
Allen RD
影响因子：
2.9
作者：
GAFFAL, KP;ELGAMMAL, S
通讯作者：
ELGAMMAL, S
DOI：
10.1073/pnas.85.23.8998
发表时间：
1988-12-01
影响因子：
11.1
作者：
FROHMAN, MA;DUSH, MK;MARTIN, GR
通讯作者：
MARTIN, GR