Art of Making Artificial Liver: Depicting Human Liver Biology and Diseases in Mice.
Art of Making Artificial Liver: Depicting Human Liver Biology and Diseases in Mice.
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DOI：
10.1055/s-0040-1701444
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发表时间：
2020-05
影响因子：
4.2
通讯作者：
Saito T
中科院分区：
文献类型：
--
作者：
Sugahara G;Ishida Y;Sun J;Tateno C;Saito T
随着肝脏生物工程和细胞生物学的进步，2001年建立了第一代人源化肝脏嵌合小鼠（HLCM）模型。HLCM系统最初是为了满足乙型肝炎病毒和丙型肝炎病毒感染研究对方便且具有生理代表性的小动物模型的需求而开发的。在过去的二十年中，HLCM 系统在质量、生产能力和实用性方面有了很大的发展，从而使其多功能性超出了病毒性肝炎的研究范围。因此，它越来越多地用于各种应用，包括但不限于药物代谢和药代动力学以及干细胞生物学的研究。迄今为止，已经建立了十多个各具特色的HLCM系统，每个模型系统都有相似之处，也有独特的特点，这往往让最终用户感到困惑。因此，本综述旨在总结每个模型系统的历史、演变、优点和缺陷，目的是为研究人员实现理想的 HLCM 系统提供必要的全面信息。此外，这篇综述文章总结了 HLCM 及其衍生物对我们对各种人类肝脏疾病的机制理解的贡献、其新应用的潜力及其当前的局限性。
Advancement in both bioengineering and cell biology of the liver led to the establishment of the first-generation humanized liver chimeric mouse (HLCM) model in 2001. The HLCM system was initially developed to satisfy the necessity for a convenient and physiologically representative small animal model for studies of hepatitis B virus and hepatitis C virus infection. Over the last two decades, the HLCM system has substantially evolved in quality, production capacity, and utility, thereby growing its versatility beyond the study of viral hepatitis. Hence, it has been increasingly employed for a variety of applications including, but not limited to, the investigation of drug metabolism and pharmacokinetics and stem cell biology. To date, more than a dozen distinctive HLCM systems have been established, and each model system has similarities as well as unique characteristics, which are often perplexing for end-users. Thus, this review aims to summarize the history, evolution, advantages, and pitfalls of each model system with the goal of providing comprehensive information that is necessary for researchers to implement the ideal HLCM system for their purposes. Furthermore, this review article summarizes the contribution of HLCM and its derivatives to our mechanistic understanding of various human liver diseases, its potential for novel applications, and its current limitations.
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影响因子：
6.7
作者：
Bility MT;Cheng L;Zhang Z;Luan Y;Li F;Chi L;Zhang L;Tu Z;Gao Y;Fu Y;Niu J;Wang F;Su L
通讯作者：
Su L
影响因子：
46.9
作者：
Azuma, Hisaya;Paulk, Nicole;Grompe, Markus
通讯作者：
Grompe, Markus
DOI：
10.1177/1535370214531872
发表时间：
2014-09
期刊：
Experimental biology and medicine (Maywood, N.J.)
影响因子：
--
作者：
Bale SS;Vernetti L;Senutovitch N;Jindal R;Hegde M;Gough A;McCarty WJ;Bakan A;Bhushan A;Shun TY;Golberg I;DeBiasio R;Usta BO;Taylor DL;Yarmush ML
通讯作者：
Yarmush ML
影响因子：
1.8
作者：
Barnes, Alan J.;Baker, David R.;Wilson, Ian D.
通讯作者：
Wilson, Ian D.
DOI：
10.1016/j.biocel.2009.03.001
发表时间：
2009-08-01
影响因子：
4
作者：
Atzori, Luigi;Poli, Giuseppe;Perra, Andrea
通讯作者：
Perra, Andrea