Response of the bacteriophage T4 replisome to noncoding lesions and regression of a stalled replication fork.
Response of the bacteriophage T4 replisome to noncoding lesions and regression of a stalled replication fork.
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DOI：
10.1016/j.jmb.2010.06.027
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发表时间：
2010-09-03
影响因子：
5.6
通讯作者：
Benkovic SJ
中科院分区：
文献类型：
--
作者：
Nelson SW;Benkovic SJ
DNA不断地受到内源性和外源性因素的破坏。由此产生的DNA损伤有可能阻止复制体的进展，可能导致复制叉崩溃。在这里，我们检查了前导或滞后链模板中的非编码DNA损伤对噬菌体T4复制体的影响。滞后链模板中受损的碱基不会影响复制叉子的进程。相反，停滞不前的滞后链聚合酶从病变中回收，并从受损碱基上游启动新冈崎片段的合成。相反，当复制体遇到前导链模板中的阻断病变时，复制叉在完全崩溃之前仅移动约1kb超出DNA病变点。在此期间，原始体和滞后链聚合酶保持活跃，冈崎片段在领先链损伤的点之外合成。在前导链模板上没有新的启动事件的证据。相反，由停滞的复制叉产生的DNA结构是DNA修复解旋酶UvsW的底物。UvsW催化一个停滞不前的复制叉子回归到一个“鸡脚”结构，该结构被认为是无错误损伤旁路的中间环节。
DNA is constantly damaged by endogenous and exogenous agents. The resulting DNA lesions have the potential to halt the progression of the replisome, possibly leading to replication fork collapse. Here, we examine the effect of a non-coding DNA lesion in either the leading or lagging strand template on the bacteriophage T4 replisome. A damaged base in the lagging strand template does not affect the progression of the replication fork. Instead, the stalled lagging strand polymerase recycles from the lesion and initiates synthesis of the new Okazaki fragment upstream from the damaged base. In contrast, when the replisome encounters a blocking lesion in the leading strand template, the replication fork only travels approximately 1 kb beyond the point of the DNA lesion before complete replication fork collapse. The primosome and lagging strand polymerase remain active during this period and an Okazaki fragment is synthesized beyond the point of the leading strand lesion. There is no evidence for a new priming event on the leading strand template. Instead, the DNA structure that is produced by the stalled replication fork is a substrate for the DNA repair helicase, UvsW. UvsW catalyzes the regression of a stalled replication fork into a “chicken foot” structure that has been postulated to be an intermediate in an error-free lesion bypass pathway.
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影响因子：
4.8
作者：
McInerney, Peter;O'Donnell, Mike
通讯作者：
O'Donnell, Mike
影响因子：
64.8
作者：
Cox, MM;Goodman, MF;Marians, KJ
通讯作者：
Marians, KJ
影响因子：
4.8
作者：
McInerney, P;O'Donnell, M
通讯作者：
O'Donnell, M
DOI：
10.1073/pnas.121009398
发表时间：
2001-07-17
影响因子：
11.1
作者：
Jones, CE;Mueser, TC;Nossal, NG
通讯作者：
Nossal, NG
影响因子：
4.8
作者：
Kadyrov, FA;Drake, JW
通讯作者：
Drake, JW