Dissociation of sarcoglycans and the dystrophin carboxyl terminus from the sarcolemma in enteroviral cardiomyopathy.
Dissociation of sarcoglycans and the dystrophin carboxyl terminus from the sarcolemma in enteroviral cardiomyopathy.
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肠道病毒性心肌病中肌聚糖和肌营养不良蛋白羧基末端从肌膜解离。
DOI：
10.1161/01.res.87.6.489
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发表时间：
2000
影响因子：
20.1
通讯作者：
Knowlton,KU
中科院分区：
文献类型：
--
作者：
Lee,GH;Badorff,C;Knowlton,KU
—Enteroviral infection can cause an acquired form of dilated cardiomyopathy. We recently reported that dystrophin is cleaved, functionally impaired, and morphologically disrupted in vitro as well as in vivo during infection with coxsackievirus B3. Genetic dystrophin truncations lead to a marked decrease in dystrophin-associated glycoproteins, whereas expression of only the naturally occurring dystrophin carboxyl terminus, Dp-71, restores the sarcolemmal association of the dystrophin-associated glycoproteins. We sought to determine whether acute cleavage of dystrophin leads to a dissociation of the carboxyl-terminal dystrophin fragment and of the sarcoglycans from the sarcolemma during coxsackievirus B3 infection. We found that in cultured cardiac myocytes and murine hearts infected with coxsackievirus B3, the sarcolemmal localization of the dystrophin carboxyl terminus is lost. The dystrophin-associated glycoproteins α-, β-, γ-, and δ-sarcoglycan and β-dystroglycan were markedly decreased in the membrane fraction of infected cells in culture, and the typical sarcolemmal localization for each of these proteins was lost in coxsackievirus-B3–infected cardiomyocytes in vivo. Furthermore, sucrose gradient ultracentrifugation demonstrated that δ-sarcoglycan was physically dissociated from dystrophin within the membrane fraction. In vivo, the sarcolemmal integrity was functionally impaired with Evans blue dye uptake even though there was no generalized disruption of the sarcolemma of infected myocytes evidenced by intact wheat germ agglutinin staining. In analogy to hereditary sarcoglycanopathies, this disintegration of the sarcoglycan complex may, in addition to the dystrophin cleavage, play an important role in the pathogenesis of enterovirus-induced cardiomyopathy. These results imply a potential role for disruption of the sarcoglycans in an acquired form of heart failure.
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影响因子：
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作者：
Ball,ED;Fanger,MW
通讯作者：
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影响因子：
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作者：
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DOI：
10.1073/pnas.79.17.5374
发表时间：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1056/nejm198706113162402
发表时间：
1987
期刊：
The New England journal of medicine
影响因子：
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作者：
Takvorian,T;Canellos,GP;Ritz,J;Freedman,AS;Anderson,KC;Mauch,P;Tarbell,N;Coral,F;Daley,H;Yeap,B
通讯作者：
Yeap,B
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影响因子：
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作者：
Clift,RA;Buckner,CD;Thomas,ED;Kopecky,KJ;Appelbaum,FR;Tallman,M;Storb,R;Sanders,J;Sullivan,K;Banaji,M
通讯作者：
Banaji,M