Transcriptional control of local auxin distribution by the CsDFB1-CsPHB module regulates floral organogenesis in cucumber.
Transcriptional control of local auxin distribution by the CsDFB1-CsPHB module regulates floral organogenesis in cucumber.
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DOI：
10.1073/pnas.2023942118
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发表时间：
2021-02-23
影响因子：
11.1
通讯作者：
Sui X
中科院分区：
文献类型：
--
作者：
Nie J;Shan N;Liu H;Yao X;Wang Z;Bai R;Guo Y;Duan Y;Wang C;Sui X
生长素是一种重要的植物激素，对植物发育的许多方面都有重要影响，包括分生组织的维持、原基的发生、花器官的形成和维管的分化。生长素的局部生物合成和极性运输对于建立和维持生长素梯度以确保植物正常发育是必不可少的。在这里，我们证明，CsDFB 1，植物半胱氨酸蛋白酶抑制剂超家族的成员，这是以前牵连在防御反应，在调节当地生长素的分布，从而影响黄瓜花器官发生中起着至关重要的作用。遗传和生化分析表明，CsDFB 1影响当地生长素分布作为衰减剂，与CsPHB相互作用，并调节CsPHB介导的CsYUC 2和CsPIN 1的转录控制。我们的研究结果揭示了微调本地生长素在植物中的分布。植物半胱氨酸蛋白酶抑制剂是一种半胱氨酸蛋白酶抑制剂，在植物防御反应中发挥重要作用。在这项工作中，我们描述了一个意想不到的作用，半胱氨酸蛋白酶抑制剂样蛋白变形花芽1（CsDFB 1）作为一个转录调节本地生长素分布在黄瓜（黄瓜L.）。CsDFB 1在花的分生组织、花原基和维管系统中有强表达。RNA干扰（RNAi）介导的沉默CsDFB 1导致显着增加的花器官和维管束的数量，连同显着积累的生长素。相反，伴随着生长素的减少，CsDFB 1的过表达导致花器官数量的急剧减少和维管图案的明显缺陷，以及器官融合。CsDFB 1与PHABULOSA（CsPHB）的黄瓜直系同源物发生物理相互作用，PHABULOSA是一种HD-ZIP III转录因子，其转录物表现出与CsDFB 1相同的模式。CsPHB的过表达增加了茎尖中生长素的积累，并诱导了与CsDFB 1-RNAi株系相似的成花表型。此外，遗传和生化分析表明，CsDFB 1损害CsPHB介导的转录调控的生长素生物合成基因YUCCA 2和生长素外排载体PIN-FORMED 1，因此在生长素分布中起着关键作用。综上所述，我们认为CsDFB 1-CsPHB模块代表了黄瓜花器官发生和维管分化中生长素局部分布的调控途径。
Auxin is a key phytohormone influencing multiple aspects of plant development, including meristem maintenance, primordia initiation, floral organogenesis, and vascular differentiation. Local auxin biosynthesis and polar auxin transport are essential to establish and maintain auxin gradients that ensure proper plant development. Here, we demonstrate that CsDFB1, a member of the plant cystatin superfamily, which was previously implicated in defense responses, plays a critical role in regulating local auxin distribution and thus influences floral organogenesis in cucumber. Genetic and biochemical assays suggest that CsDFB1 affects local auxin distribution by acting as an attenuator that interacts with CsPHB and modulates CsPHB-mediated transcriptional control of CsYUC2 and CsPIN1. Our results shed light on the fine tuning of local auxin distribution in plants. Plant cystatins are cysteine proteinase inhibitors that play key roles in defense responses. In this work, we describe an unexpected role for the cystatin-like protein DEFORMED FLORAL BUD1 (CsDFB1) as a transcriptional regulator of local auxin distribution in cucumber (Cucumis sativus L.). CsDFB1 was strongly expressed in the floral meristems, floral primordia, and vasculature. RNA interference (RNAi)-mediated silencing of CsDFB1 led to a significantly increased number of floral organs and vascular bundles, together with a pronounced accumulation of auxin. Conversely, accompanied by a decrease of auxin, overexpression of CsDFB1 resulted in a dramatic reduction in floral organ number and an obvious defect in vascular patterning, as well as organ fusion. CsDFB1 physically interacted with the cucumber ortholog of PHABULOSA (CsPHB), an HD-ZIP III transcription factor whose transcripts exhibit the same pattern as CsDFB1. Overexpression of CsPHB increased auxin accumulation in shoot tips and induced a floral phenotype similar to that of CsDFB1-RNAi lines. Furthermore, genetic and biochemical analyses revealed that CsDFB1 impairs CsPHB-mediated transcriptional regulation of the auxin biosynthetic gene YUCCA2 and the auxin efflux carrier PIN-FORMED1, and thus plays a pivotal role in auxin distribution. In summary, we propose that the CsDFB1-CsPHB module represents a regulatory pathway for local auxin distribution that governs floral organogenesis and vascular differentiation in cucumber.
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影响因子：
4.5
作者：
Chang S;Chen Y;Jia S;Li Y;Liu K;Lin Z;Wang H;Chu Z;Liu J;Xi C;Zhao H;Han S;Wang Y
通讯作者：
Wang Y
影响因子：
4.5
作者：
Fàbregas N;Formosa-Jordan P;Confraria A;Siligato R;Alonso JM;Swarup R;Bennett MJ;Mähönen AP;Caño-Delgado AI;Ibañes M
通讯作者：
Ibañes M
影响因子：
9.2
作者：
Eshed, Y;Baum, SF;Bowman, JL
通讯作者：
Bowman, JL
影响因子：
6.4
作者：
Brumos, Javier;Alonso, Jose M.;Stepanova, Anna N.
通讯作者：
Stepanova, Anna N.
影响因子：
4.6
作者：
Kelley, Dior R.;Arreola, Alexandra;Gasser, Charles S.
通讯作者：
Gasser, Charles S.