Celastrol inhibits the proliferation and angiogenesis of high glucose-induced human retinal endothelial cells.
Celastrol inhibits the proliferation and angiogenesis of high glucose-induced human retinal endothelial cells.
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DOI：
10.1186/s12938-021-00904-5
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发表时间：
2021-06-30
影响因子：
3.9
通讯作者：
Chang X
中科院分区：
文献类型：
--
作者：
Fang J;Chang X
糖尿病视网膜病变（DR）是糖尿病最常见的微血管并发症之一。雷公藤红素对糖尿病的各种并发症有一定的改善作用。因此，本研究旨在探讨雷公藤红素是否通过下调HIF 1/VEGF信号通路抑制高糖诱导的人视网膜内皮细胞（human retinal endothelial cells，hRECs）增殖和血管生成。MTT法检测葡萄糖、雷公藤红素和二甲基乙氧烯丙基甘氨酸（DMOG）对hRECs活力和增殖的影响。用transwell法和微管形成法检测葡萄糖、雷公藤红素和DMOG处理的hRECs的侵袭能力和微管形成能力。采用Western blot和RT-qPCR分析hRECs中HIF-1 α和VEGF的表达，免疫荧光法检测hRECs中ICAM-1的表达。HG诱导可促进hRECs的增殖、侵袭和管腔形成能力，增加HIF-1α和VEGF的表达，雷公藤红素在0.5 ~ 2.0 μM范围内逐渐抑制其表达。DMOG被认为是HIF 1 α激动剂，其减弱雷公藤红素对HG诱导的hREC的作用。 雷公藤红素通过下调HIF 1 α/VEGF信号通路抑制HG诱导的hRECs增殖和血管生成。
Diabetic retinopathy (DR) is one of the most common microvascular complications of diabetes. Celastrol plays a certain role in the improvement of various diabetes complications. Therefore, this study aimed to explore whether celastrol inhibited the proliferation and angiogenesis of high glucose (HG)-induced human retinal endothelial cells (hRECs) by down-regulating the HIF1/VEGF signaling pathway. The viability and proliferation of hRECs treated with glucose, celastrol or dimethyloxallyl glycine (DMOG) were analyzed by MTT assay. The invasion and tube formation ability of hRECs treated with glucose, celastrol or DMOG were in turn detected by transwell assay and tube formation assay. The expression of HIF1α and VEGF in hRECs after indicated treatment was analyzed by Western blot analysis and RT-qPCR analysis and ICAM-1 expression in hRECs after indicated treatment was detected by immunofluorescence assay HG induction promoted the proliferation, invasion and tube formation ability and increased the expression of HIF-1α and VEGF of hRECs, which were gradually suppressed by celastrol changing from 0.5 to 2.0 μM. DMOG was regarded as a HIF1α agonist, which attenuated the effect of celastrol on HG-induced hRECs. Celastrol inhibited the proliferation and angiogenesis of HG-induced hRECs by down-regulating the HIF1α/VEGF signaling pathway.
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影响因子：
--
作者：
Lee YM;Kim CS;Jo K;Sohn EJ;Kim JS;Kim J
通讯作者：
Kim J
影响因子：
8.2
作者：
Lin M;Chen Y;Jin J;Hu Y;Zhou KK;Zhu M;Le YZ;Ge J;Johnson RS;Ma JX
通讯作者：
Ma JX
雷公藤红醇通过 PI3K/AKT 通路减缓大鼠早期糖尿病肾病的进展
影响因子：
3.9
作者：
Nie Y;Fu C;Zhang H;Zhang M;Xie H;Tong X;Li Y;Hou Z;Fan X;Yan M
通讯作者：
Yan M
影响因子：
5.4
作者：
Guan Y;Cui ZJ;Sun B;Han LP;Li CJ;Chen LM
通讯作者：
Chen LM
影响因子：
5
作者：
Li, H;Zhang, YY;Li, D
通讯作者：
Li, D