Disruption of the cytoskeleton during Semaphorin 3A induced growth cone collapse correlates with differences in actin organization and associated binding proteins.
Disruption of the cytoskeleton during Semaphorin 3A induced growth cone collapse correlates with differences in actin organization and associated binding proteins.
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DOI：
10.1002/dneu.20732
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发表时间：
2009-09-01
影响因子：
3
通讯作者：
Bridgman, Paul C.
中科院分区：
文献类型：
--
作者：
Brown, Jacquelyn A.;Bridgman, Paul C.
排斥性引导线索诱导生长锥塌陷或塌陷和缩回。崩溃的结果是破坏和损失的肌动蛋白细胞骨架。生长锥中富含肌动蛋白的区域含有影响纤维组织的结合蛋白，如Arp 2/3、corptin和fascin，但对这些蛋白在塌陷中的作用知之甚少。在这里，我们表明，Semaphorin 3A（Sema 3A），这是排斥小鼠背根神经节神经元，肌动蛋白结合蛋白和它们相关的丝有不平等的影响。相对于总蛋白，阿普-2和corcin的免疫荧光染色强度降低，而在未提取的生长锥中，fascin增加。肌成束蛋白和肌球蛋白IIB染色重新分布并显示重叠增加。肌动蛋白丝在塌陷过程中的损失程度与旋转阴影电子显微镜检测到的丝超结构相关。折叠导致分支的f-肌动蛋白网络的损失，而肌动蛋白束在不同程度上被部分保留。结合已知的Sema 3A对肌动蛋白的影响，这表明了一种遵循以下顺序的塌陷模型：肌动蛋白网状物解聚，然后是肌成束蛋白相关肌动蛋白束的部分解聚，以及它们向神经突的运动以完全塌陷。重新定位的肌成束蛋白相关的肌动蛋白束可能提供底物的肌动球蛋白收缩，产生回缩。
Repulsive guidance cues induce growth cone collapse or collapse and retraction. Collapse results from disruption and loss of the actin cytoskeleton. Actin rich regions of growth cones contain binding proteins that influence filament organization, such as Arp2/3, cortactin, and fascin, but little is known about the role that these proteins play in collapse. Here we show that Semaphorin 3A (Sema 3A), which is repulsive to mouse dorsal root ganglion neurons, has unequal effects on actin binding proteins and their associated filaments. The immunofluorescence staining intensity of Arp-2 and cortactin decreases relative to total protein, while in unextracted growth cones fascin increases. Fascin and myosin IIB staining redistribute and show increased overlap. The degree of actin filament loss during collapse correlates with filament superstructures detected by rotary shadow electron microscopy. Collapse results in the loss of branched f-actin meshworks, while actin bundles are partially retained to varying degrees. Taken together with the known affects of Sema 3A on actin, this suggests a model for collapse that follows a sequence; depolymerization of actin meshworks followed by partial depolymerization of fascin associated actin bundles and their movement to the neurite to complete collapse. The relocated fascin associated actin bundles may provide the substrate for actomyosin contractions that produce retraction.
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影响因子：
7.8
作者：
Nemethova, Maria;Auinger, Sonja;Small, J. Victor
通讯作者：
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影响因子：
3.3
作者：
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DOI：
10.1083/jcb.121.4.867
发表时间：
1993-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
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通讯作者：
Raper JA
影响因子：
4
作者：
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通讯作者：
Borisy, Gary G.
影响因子：
3.3
作者：
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通讯作者：
Kolega, John