Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella.
Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella.
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DOI：
10.1083/jcb.200709134
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发表时间：
2008-03-24
影响因子：
7.8
通讯作者：
Small, J. Victor
中科院分区：
文献类型：
--
作者：
Nemethova, Maria;Auinger, Sonja;Small, J. Victor
丝状伪足是杆状延伸，通常在细胞迁移中起引导作用。我们现在在鱼类成纤维细胞中证明，丝状伪足在迁移前沿后面的片层区域中产生收缩束方面发挥着重要作用。通过含有桩蛋白的焦点复合物与基底形成粘附的丝状伪足将其近端部分贡献给应力纤维组装，而横向折叠的丝状伪足有助于构建平行于细胞边缘的收缩束。标记肌动蛋白和肌成束蛋白的细胞的相关光学和电子显微镜证实了丝状伪足束整合到板层网络中。肌球蛋白 II 的抑制并没有抑制丝状伪足的波动和折叠运动或它们进入薄片，但丝状伪足随后没有整合到收缩阵列中。 B16 黑色素瘤细胞获得了类似的结果。这些和其他发现支持以下观点：丝状伪足和片状伪足中产生的用于突出的丝被回收用于播种肌动球蛋白阵列以用于回缩。
Filopodia are rodlike extensions generally attributed with a guidance role in cell migration. We now show in fish fibroblasts that filopodia play a major role in generating contractile bundles in the lamella region behind the migrating front. Filopodia that developed adhesion to the substrate via paxillin containing focal complexes contributed their proximal part to stress fiber assembly, and filopodia that folded laterally contributed to the construction of contractile bundles parallel to the cell edge. Correlated light and electron microscopy of cells labeled for actin and fascin confirmed integration of filopodia bundles into the lamella network. Inhibition of myosin II did not subdue the waving and folding motions of filopodia or their entry into the lamella, but filopodia were not then integrated into contractile arrays. Comparable results were obtained with B16 melanoma cells. These and other findings support the idea that filaments generated in filopodia and lamellipodia for protrusion are recycled for seeding actomyosin arrays for use in retraction.
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