The ubiquitin conjugating enzyme, TaU4 regulates wheat defence against the phytopathogen Zymoseptoria tritici.
The ubiquitin conjugating enzyme, TaU4 regulates wheat defence against the phytopathogen Zymoseptoria tritici.
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DOI：
10.1038/srep35683
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发表时间：
2016-10-19
影响因子：
4.6
通讯作者：
Sadanandom A
中科院分区：
文献类型：
--
作者：
Millyard L;Lee J;Zhang C;Yates G;Sadanandom A
稻瘟病菌（Mycosphaerella graminicola, Zymoseptoria tritici，俗称Septoria）是稻瘟病（Septoria Leaf Blotch， STB）的致病因子，被认为是欧洲小麦生产的主要威胁之一。以前的研究已经表明泛素化在植物防御多种病原体中的重要性。然而，小麦的泛素化机制仍在研究中，特别是E2酶具有控制泛素化的能力，从而控制许多不同靶蛋白的命运。在本研究中，我们鉴定了一种E2酶，即Triticum aestivum Ubiquitin偶联酶4 (TaU4)，该酶在小麦抵御稻瘟病中起作用。我们通过E2充电实验证明了TaU4是一种真正的E2酶。TaU4定位于细胞质和细胞核中，因此可能在多个区室中与E3连接酶和底物蛋白相互作用。病毒诱导的小麦叶片中TaU4基因沉默导致疾病症状发展延迟，室间隔菌生长和繁殖减少。我们得出结论，TaU4是一种新的抗Septoria负调节因子。
Mycosphaerella graminicola (Zymoseptoria tritici commonly known as Septoria), the causal agent of Septoria Leaf Blotch (STB), is considered one of the major threats to European wheat production. Previous studies have shown the importance of ubiquitination in plant defence against a multitude of pathogens. However the ubiquitination machinery in wheat is under studied, particularly E2 enzymes that have the ability to control the ubiquitination and thereby the fate of many different target proteins. In this study we identify an E2 enzyme, Triticum aestivum Ubiquitin conjugating enzyme 4 (TaU4) that functions in wheat defence against Septoria. We demonstrate TaU4 to be a bona fide E2 enzyme through an E2 charging assay. TaU4 localises in both the cytoplasm and nucleus, therefore potentially interacting with E3 ligases and substrate proteins in multiple compartments. Virus Induced Gene Silencing of TaU4 in wheat leaves resulted in delayed development of disease symptoms, reduced Septoria growth and reproduction. We conclude that TaU4 is a novel negative regulator of defence against Septoria.
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DOI：
10.1093/bioinformatics/8.3.275
发表时间：
1992-06-01
期刊：
COMPUTER APPLICATIONS IN THE BIOSCIENCES
影响因子：
--
作者：
JONES, DT;TAYLOR, WR;THORNTON, JM
通讯作者：
THORNTON, JM
影响因子：
3
作者：
Löytynoja A;Goldman N
通讯作者：
Goldman N
影响因子：
4.3
作者：
Gamini R;Han W;Stone JE;Schulten K
通讯作者：
Schulten K
影响因子：
64.5
作者：
Koegl, M;Hoppe, T;Jentsch, S
通讯作者：
Jentsch, S
影响因子：
4.8
作者：
Jensen, JP;Bates, PW;Weissman, AM
通讯作者：
Weissman, AM