Novel rat model of tympanostomy tube otorrhea.
Novel rat model of tympanostomy tube otorrhea.
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DOI：
10.1016/j.ijporl.2011.11.001
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发表时间：
2012-02
影响因子：
1.5
通讯作者：
Hebda, Patricia A.
中科院分区：
文献类型：
--
作者：
Silva, Rodrigo C.;Dohar, Joseph E.;Hebda, Patricia A.
鼓膜造口置管术最常见的并发症是鼓膜造口置管术后中耳炎（TTO）。没有研究描述了TTO的可重现动物模型。我们的目的是建立一种大鼠TTO模型，该模型可用于检测整个感染过程中TNF-α和IL-1β的水平。用牙胶堵塞55只雄性Sprague-Dawley白化病大鼠的左咽鼓管（ETO=咽鼓管堵塞）。通过每周一次的耳显微镜检查确定中耳（ME）积液。在3周时，双侧放置鼓膜造口管，并通过管用肺炎链球菌双侧接种ME。将大鼠随机分配至两种每日耳局部治疗之一：环丙沙星/地塞米松（CDX）或安慰剂。将两个给药组中的动物进一步分为接受1、2、5或7天给药。治疗结束后处死大鼠。观察两组中耳炎发生率、中耳培养阳性率及中耳液中TNF-α、IL-1β水平。左ETO，然后ME接种S。肺炎和安慰剂治疗导致持续感染（10天时100%培养阳性ME液）和耳炎（85.7%）。左耳持续感染伴IL-1β和TNF-α水平显著升高。CDX治疗的耳在所有时间点的耳畸形发生率均较低，IL-1β和TNF-α水平也较低。本研究首次描述了急性TTO的可重复动物模型。手术阻塞ET，然后放置TT和ME接种S。肺炎引起持续性耳炎和感染。IL-1β和TNF-α可能是持续性中耳感染的潜在标志物。这种新的模型可能用于TTO的发病机制和治疗的未来研究。
Tympanostomy tube otorrhea (TTO), caused by the presence of pathogenic bacteria in the middle ear, is the most common complication of TT insertion. No studies have described a reproducible animal model of TTO. We aimed to develop a rat model of TTO which, in turn, could be used to assay the levels of TNF-α and IL-1β through the course of the infection. The left Eustachian tubes of 55 male Sprague-Dawley albino rats were occluded with gutta-percha (ETO=Eustachian Tube Occlusion). Middle ear (ME) effusion was ascertained by weekly otomicroscopy. At 3 weeks tympanostomy tubes were placed bilaterally and the MEs were inoculated bilaterally with Streptococcus pneumoniae through the tubes. The rats were randomly assigned to one of two daily ototopical treatments: ciprofloxacin/dexamethasone (CDX) or placebo. The animals in each of the two treatment groups were further divided to receive 1, 2, 5 or 7 days of treatment. The rats were sacrificed after treatment was finished. The rates of otorrhea, positive middle ear (ME) cultures, and levels of TNF-α and IL-1β in the ME fluid were measured. Left ETO followed by ME inoculation with S. pneumoniae and treatment with placebo resulted in persistent infection (100% culture-positive ME fluid at 10 days) and otorrhea (85.7%). Persistent infection of the left ear was accompanied by significantly elevated the levels of IL-1β and TNF-α. Ears treated with CDX had lower rates of otorrhea at all time points and lower levels of IL-1β and TNF-α. This study is the first to describe a reproducible animal model of acute TTO. Surgical obstruction of the ET, followed by TT placement and ME inoculation with S. pneumoniae induced persistent otorrhea and infection. Both IL-1β and TNF-α appear to be potential markers of persistent middle ear infection. This novel model may be used in future studies of the pathogenesis and therapy of TTO.
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影响因子：
2.5
作者：
HERMANSSON, A;EMGARD, P;HELLSTROM, S
通讯作者：
HELLSTROM, S
DOI：
10.1016/s0165-5876(97)00147-x
发表时间：
1998-01-01
影响因子：
1.5
作者：
Bluestone, CD
通讯作者：
Bluestone, CD
影响因子：
1.4
作者：
MANDEL, EM;CASSELBRANT, ML;KURSLASKY, M
通讯作者：
KURSLASKY, M
影响因子：
11.8
作者：
Ruohola, Aino;Meurman, Olli;Ruuskanen, Olli
通讯作者：
Ruuskanen, Olli
DOI：
10.1016/j.ijporl.2003.08.043
发表时间：
2003-12-01
影响因子：
1.5
作者：
Dohar, J
通讯作者：
Dohar, J