RNA degradation is required for the germ-cell to maternal transition in Drosophila.
RNA degradation is required for the germ-cell to maternal transition in Drosophila.
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DOI：
10.1016/j.cub.2021.04.052
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发表时间：
2021-07-26
期刊：
影响因子：
--
通讯作者：
Rangan P
中科院分区：
文献类型：
--
作者：
Blatt P;Wong-Deyrup SW;McCarthy A;Breznak S;Hurton MD;Upadhyay M;Bennink B;Camacho J;Lee MT;Rangan P
在有性繁殖的动物中，卵母细胞提供了大量的rna，这些rna对受精后的发育至关重要。在卵子发生过程中调控母体RNA组成的机制尚不清楚。在这里，我们发现在卵发生的早期阶段表达的rna子集在卵母细胞规范过程中受到调节降解。不能去除这些rna会导致卵母细胞功能障碍和死亡。我们通过靶向降解未分化生殖细胞中表达的特定rna，确定了rna降解超级杀手复合物和No-Go衰变因子Pelota作为卵子发生的关键调节因子。这些调节因子的目标rna富集胞苷序列，由多聚嘧啶束结合蛋白半品脱结合。因此，RNA降解有助于协调生殖细胞到母体的转变，从而产生母体对受精卵的贡献。Blatt等人发现，当指定卵母细胞时，生殖细胞中表达的一组rna是No Go衰变途径组分介导的受调节降解的靶标。他们发现这种受调控的RNA降解对于正常的卵母细胞发育和生育至关重要。
In sexually reproducing animals, the oocyte contributes a large supply of RNAs that are essential to launch development upon fertilization. The mechanisms that regulate the composition of the maternal RNA contribution during oogenesis are unclear. Here, we show that a subset of RNAs expressed during the early stages of oogenesis is subjected to regulated degradation during oocyte specification. Failure to remove these RNAs results in oocyte dysfunction and death. We identify the RNA-degrading Super Killer complex and No-Go Decay factor Pelota as key regulators of oogenesis via targeted degradation of specific RNAs expressed in undifferentiated germ cells. These regulators target RNAs enriched for cytidine sequences that are bound by the polypyrimidine tract binding protein Half pint. Thus, RNA degradation helps orchestrate a germ cell-to-maternal transition that gives rise to the maternal contribution to the zygote. Blatt et al. find that a cohort of RNAs expressed in germ cells are targeted for regulated degradation mediated by components of the No Go Decay pathway when oocytes are specified. They find that this regulated RNA degradation is essential for proper oocyte development and fertility.
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DOI：
10.1093/bioinformatics/btr064
发表时间：
2011-04-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Grant CE;Bailey TL;Noble WS
通讯作者：
Noble WS
影响因子：
3.7
作者：
Edri S;Tuller T
通讯作者：
Tuller T
核糖体分析揭示了果蝇果蝇中普遍存在的终止密码子读取。
影响因子：
7.7
作者：
Dunn JG;Foo CK;Belletier NG;Gavis ER;Weissman JS
通讯作者：
Weissman JS
影响因子：
64.5
作者：
Halbach, Felix;Reichelt, Peter;Conti, Elena
通讯作者：
Conti, Elena
影响因子：
5.6
作者：
Fuchs, Gabriele;Diges, Camille;Sarnow, Peter
通讯作者：
Sarnow, Peter