Mapping QTL Contributing to Variation in Posterior Lobe Morphology between Strains of Drosophila melanogaster.
Mapping QTL Contributing to Variation in Posterior Lobe Morphology between Strains of Drosophila melanogaster.
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绘制QTL导致果蝇菌株之间后叶形态变化的变化。
DOI：
10.1371/journal.pone.0162573
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Macdonald SJ
中科院分区：
文献类型：
--
作者：
Hackett JL;Wang X;Smith BR;Macdonald SJ
密切相关的，否则形态相似的昆虫物种经常表现出显着的分歧的形状和/或大小的雄性生殖器结构，一种现象被认为是由性选择驱动。种间比较研究可以帮助阐明作用于生殖器结构的进化力量，以推动这种快速分化。然而，物种间性性状差异的遗传解剖经常受到种间重组体产生困难的阻碍。种内变异可以用来研究快速进化的性特征的遗传学，在这里，我们对D.黑腹菌叶是雄性特有的突起，从D. melanogaster和三个密切相关的物种，是交配所必需的，并显示出跨物种的形式根本分歧。在物种内，叶的形状和大小也有丰富的变异，虽然这种变异比物种间的变异要微妙得多，但它仍然为QTL作图提供了原材料。我们创建了一个先进的互交人口从一对表型不同的近交系，表型和基因分型后，测序的重组体，映射几个QTL有助于叶形态的各种措施。在我们的作图群体中的额外世代的交换导致QTL间隔小于F2作图设计的典型间隔。我们映射的间隔与我们以前在一个独立的映射交叉中确定的一对叶QTL重叠，可能表明性状变异的共享遗传控制水平。我们的QTL还涉及一套基因，已被证明有助于后叶的发展。这些基因座是强有力的候选人窝藏自然分离的网站，有助于表型变异内D。黑腹的，也可能是那些有助于分歧叶形态物种之间。
Closely-related, and otherwise morphologically similar insect species frequently show striking divergence in the shape and/or size of male genital structures, a phenomenon thought to be driven by sexual selection. Comparative interspecific studies can help elucidate the evolutionary forces acting on genital structures to drive this rapid differentiation. However, genetic dissection of sexual trait divergence between species is frequently hampered by the difficulty generating interspecific recombinants. Intraspecific variation can be leveraged to investigate the genetics of rapidly-evolving sexual traits, and here we carry out a genetic analysis of variation in the posterior lobe within D. melanogaster. The lobe is a male-specific process emerging from the genital arch of D. melanogaster and three closely-related species, is essential for copulation, and shows radical divergence in form across species. There is also abundant variation within species in the shape and size of the lobe, and while this variation is considerably more subtle than that seen among species, it nonetheless provides the raw material for QTL mapping. We created an advanced intercross population from a pair of phenotypically-different inbred strains, and after phenotyping and genotyping-by-sequencing the recombinants, mapped several QTL contributing to various measures of lobe morphology. The additional generations of crossing over in our mapping population led to QTL intervals that are smaller than is typical for an F2 mapping design. The intervals we map overlap with a pair of lobe QTL we previously identified in an independent mapping cross, potentially suggesting a level of shared genetic control of trait variation. Our QTL additionally implicate a suite of genes that have been shown to contribute to the development of the posterior lobe. These loci are strong candidates to harbor naturally-segregating sites contributing to phenotypic variation within D. melanogaster, and may also be those contributing to divergence in lobe morphology between species.
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影响因子：
2.6
作者：
Frazee, Stephen R.;Masly, John P.
通讯作者：
Masly, John P.
影响因子：
4.6
作者：
Chatterjee, Sujash S.;Uppendahl, Locke D.;Siegal, Mark L.
通讯作者：
Siegal, Mark L.
影响因子：
3.3
作者：
Cheng, Riyan;Lim, Jackie E.;Palmer, Abraham A.
通讯作者：
Palmer, Abraham A.
DOI：
10.1534/g3.111.001818
发表时间：
2012-02
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Broman KW
通讯作者：
Broman KW
影响因子：
3.3
作者：
COYNE, JA
通讯作者：
COYNE, JA