ComM is a hexameric helicase that promotes branch migration during natural transformation in diverse Gram-negative species.
ComM is a hexameric helicase that promotes branch migration during natural transformation in diverse Gram-negative species.
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DOI：
10.1093/nar/gky343
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发表时间：
2018-07-06
影响因子：
14.9
通讯作者：
Dalia AB
中科院分区：
文献类型：
--
作者：
Nero TM;Dalia TN;Wang JC;Kysela DT;Bochman ML;Dalia AB
通过自然转化获取外源DNA是多种微生物物种适应和进化的重要机制。在这里，我们描述了ComM的机制，这是一种广泛保守的AAA+蛋白，以前在自然状态的革兰氏阴性细菌物种中涉及转化DNA （tDNA）的同源重组。在体内，我们发现ComM对于霍乱弧菌和baylyacinetobacter的连锁遗传标记的有效迁移是必需的，这与分支迁移的作用是一致的。此外，ComM对于tDNA与增加的序列异源性的整合尤其重要，这表明它的活性促进了新DNA序列的获得。在体外，我们发现纯化的ComM与ssDNA结合，寡聚成六聚环，并具有双向解旋酶和分支迁移活性。基于这些数据，我们提出了自然转化过程中tDNA整合模型。这项研究为tDNA整合的神秘步骤提供了机制上的见解，并揭示了这种保守的水平基因转移机制所需的蛋白质的功能。
Acquisition of foreign DNA by natural transformation is an important mechanism of adaptation and evolution in diverse microbial species. Here, we characterize the mechanism of ComM, a broadly conserved AAA+ protein previously implicated in homologous recombination of transforming DNA (tDNA) in naturally competent Gram-negative bacterial species. In vivo, we found that ComM was required for efficient comigration of linked genetic markers in Vibrio cholerae and Acinetobacter baylyi, which is consistent with a role in branch migration. Also, ComM was particularly important for integration of tDNA with increased sequence heterology, suggesting that its activity promotes the acquisition of novel DNA sequences. In vitro, we showed that purified ComM binds ssDNA, oligomerizes into a hexameric ring, and has bidirectional helicase and branch migration activity. Based on these data, we propose a model for tDNA integration during natural transformation. This study provides mechanistic insight into the enigmatic steps involved in tDNA integration and uncovers the function of a protein required for this conserved mechanism of horizontal gene transfer.
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影响因子：
4.5
作者：
Azeroglu B;Mawer JS;Cockram CA;White MA;Hasan AM;Filatenkova M;Leach DR
通讯作者：
Leach DR
影响因子：
14.8
作者：
Kelley LA;Mezulis S;Yates CM;Wass MN;Sternberg MJ
通讯作者：
Sternberg MJ
影响因子：
3.4
作者：
Fallesen T;Roostalu J;Duellberg C;Pruessner G;Surrey T
通讯作者：
Surrey T
影响因子：
3.2
作者：
AlDeib, AA;Mahdi, AA;Lloyd, RG
通讯作者：
Lloyd, RG
影响因子：
3.2
作者：
Dalia, Ankur B.;Lazinski, David W.;Camilli, Andrew
通讯作者：
Camilli, Andrew