Ensembles of Bidirectional Kinesin Cin8 Produce Additive Forces in Both Directions of Movement.
Ensembles of Bidirectional Kinesin Cin8 Produce Additive Forces in Both Directions of Movement.
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DOI：
10.1016/j.bpj.2017.09.006
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发表时间：
2017-11-07
影响因子：
3.4
通讯作者：
Surrey T
中科院分区：
文献类型：
--
作者：
Fallesen T;Roostalu J;Duellberg C;Pruessner G;Surrey T
大多数驱动蛋白马达仅沿沿着微管向一个方向运动。驱动蛋白-5亚家族的成员最初被描述为单向加端定向马达，并显示产生皮牛顿力。然而，一些真菌驱动蛋白-5马达是双向的。双向驱动蛋白-5的力产生尚未被测量。因此，它仍然是未知的机制，非传统的负端定向运动从根本上不同于正端定向步进。使用力谱，我们在这里测量的力量，合奏纯化的芽殖酵母驱动蛋白-5 Cin 8产生的微管滑动测定在正负端方向。暂停力的相关性分析表明，单个Cin 8分子在两个运动方向上产生附加力。在集成中，Cin 8电机能够产生高达1.5pN的单电机力。因此，这些性质似乎是保守的驱动蛋白-5亚家族。力的产生在很大程度上独立于运动的方向性，表明两个方向的运动机制之间的相似性。这些结果提供了限制裂变酵母驱动蛋白-5的双向运动机制的模型的发展，并提供深入了解这种有丝分裂电机的功能。
Most kinesin motors move in only one direction along microtubules. Members of the kinesin-5 subfamily were initially described as unidirectional plus-end-directed motors and shown to produce piconewton forces. However, some fungal kinesin-5 motors are bidirectional. The force production of a bidirectional kinesin-5 has not yet been measured. Therefore, it remains unknown whether the mechanism of the unconventional minus-end-directed motility differs fundamentally from that of plus-end-directed stepping. Using force spectroscopy, we have measured here the forces that ensembles of purified budding yeast kinesin-5 Cin8 produce in microtubule gliding assays in both plus- and minus-end direction. Correlation analysis of pause forces demonstrated that individual Cin8 molecules produce additive forces in both directions of movement. In ensembles, Cin8 motors were able to produce single-motor forces up to a magnitude of ∼1.5 pN. Hence, these properties appear to be conserved within the kinesin-5 subfamily. Force production was largely independent of the directionality of movement, indicating similarities between the motility mechanisms for both directions. These results provide constraints for the development of models for the bidirectional motility mechanism of fission yeast kinesin-5 and provide insight into the function of this mitotic motor.
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DOI：
10.1083/jcb.200910125
发表时间：
2010-05-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hentrich C;Surrey T
通讯作者：
Surrey T
DOI：
10.1083/jcb.118.1.109
发表时间：
1992-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hoyt MA;He L;Loo KK;Saunders WS
通讯作者：
Saunders WS
影响因子：
3.4
作者：
HUNT, AJ;GITTES, F;HOWARD, J
通讯作者：
HOWARD, J
影响因子：
4.8
作者：
Hildebrandt, Emily R.;Gheber, Larisa;Hoyt, M. Andrew
通讯作者：
Hoyt, M. Andrew
影响因子：
64.8
作者：
Kashina, AS;Baskin, RJ;Scholey, JM
通讯作者：
Scholey, JM