GmbZIP1 negatively regulates ABA-induced inhibition of nodulation by targeting GmENOD40-1 in soybean.
GmbZIP1 negatively regulates ABA-induced inhibition of nodulation by targeting GmENOD40-1 in soybean.
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GmbZIP1 通过靶向 GmENOD40-1 负向调控 ABA 诱导的大豆结瘤抑制
DOI：
10.1186/s12870-020-02810-9
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发表时间：
2021-01-09
影响因子：
5.3
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Xu S;Song S;Dong X;Wang X;Wu J;Ren Z;Wu X;Lu J;Yuan H;Wu X;Li X;Wang Z
脱落酸（阿坝）通过阿坝信号途径在植物生长和适应中起着重要作用。ABA应答元件结合（AREB/ABF）家族转录因子是整合阿坝信号与多种信号通路的中心调节因子。长期以来，人们一直认为阿坝抑制根瘤菌的感染和根瘤的形成，但其潜在的分子机制仍然是难以捉摸的。在这里，我们表明，结瘤是非常敏感的阿坝和外源阿坝显着抑制大豆根瘤菌感染和根瘤形成。此外，我们还证明了AREB/ABF转录因子ARZIP 1在大豆的诱导和植物对阿坝的反应中都是一个主要的调节因子。ZIP 1在根瘤形成和发育过程中特异性表达。过表达ZIP 1导致根瘤菌感染减少和根瘤数量减少。此外，拟南芥ZIP 1对阿坝有反应，并且拟南芥ZIP 1的异位过表达增加了拟南芥植物在种子萌发和萌发后生长过程中对阿坝的敏感性，并赋予植物增强的耐旱性。值得注意的是，我们发现，EZIP 1直接结合到GmENOD 40 -1的启动子上，GmENOD 40 -1是根瘤形成的标志基因，可以抑制其表达。我们的研究结果确定了BZIP 1作为一个节点调节器，整合阿坝信号与抑制信号负调节结节形成。在线版本包含补充材料，可通过10.1186/s12870-020-02810-9获得。
Abscisic acid (ABA) plays an important role in plant growth and adaptation through the ABA signaling pathway. The ABA-responsive element binding (AREB/ABF) family transcriptional factors are central regulators that integrate ABA signaling with various signaling pathways. It has long been known that ABA inhibits rhizobial infection and nodule formation in legumes, but the underlying molecular mechanisms remain elusive. Here, we show that nodulation is very sensitive to ABA and exogenous ABA dramatically inhibits rhizobial infection and nodule formation in soybean. In addition, we proved that GmbZIP1, an AREB/ABF transcription factor, is a major regulator in both nodulation and plant response to ABA in soybean. GmbZIP1 was specifically expressed during nodule formation and development. Overexpression of GmbZIP1 resulted in reduced rhizobial infection and decreased nodule number. Furthermore, GmbZIP1 is responsive to ABA, and ectopic overexpression of GmbZIP1 increased sensitivity of Arabidopsis plants to ABA during seed germination and postgerminative growth, and conferred enhanced drought tolerance of plants. Remarkably, we found that GmbZIP1 directly binds to the promoter of GmENOD40–1, a marker gene for nodule formation, to repress its expression. Our results identified GmbZIP1 as a node regulator that integrates ABA signaling with nodulation signaling to negatively regulate nodule formation. The online version contains supplementary material available at 10.1186/s12870-020-02810-9.
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