Cell types and molecular architecture of the Octopus bimaculoides visual system.
Cell types and molecular architecture of the Octopus bimaculoides visual system.
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DOI：
10.1016/j.cub.2022.10.015
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发表时间：
2022-12-05
期刊：
影响因子：
9.2
通讯作者：
Niell, Cristopher M.
中科院分区：
文献类型：
--
作者：
Songco-Casey, Jeremea O.;Coffing, Gabrielle C.;Piscopo, Denise M.;Pungor, Judit R.;Kern, Andrew D.;Miller, Adam C.;Niell, Cristopher M.
食蟹猴豆荚有一个显着的视觉系统，与相机类型的眼睛和高敏锐度的视觉，他们使用的广泛复杂的视觉驱动的行为。然而，头足类动物的大脑组织与脊椎动物和无脊椎动物的大脑组织截然不同，除了神经解剖学描述之外，关于其视觉系统组织的细胞类型和分子决定因素知之甚少。在这里，我们提出了一个全面的单细胞分子图谱的章鱼视叶，这是主要的视觉处理结构的头足类动物的大脑。我们将单细胞RNA测序与RNA荧光原位杂交相结合，以确定推定的分子细胞类型，并确定其在视叶内的解剖和空间组织。我们的研究结果揭示了六个主要的神经元细胞类确定的神经递质/神经肽的使用，除了非神经元和未成熟的神经元群体。我们发现，额外的标记物将这些神经元类分为不同的解剖定位的亚型，揭示了进一步的多样性和详细的层状组织内的视叶。我们还描绘了未成熟的神经元在这个不断增长的组织到亚型定义的进化保守的发育基因，以及新的头足类和章鱼特异性基因。总之，这些研究结果概述了章鱼视觉系统的组织逻辑，基于功能决定因素，层状身份和发育标记/途径。这里展示的地图集详细介绍了章鱼视觉神经回路的“零件清单”，为研究章鱼视觉系统的发展和功能以及视觉处理的演变提供了一个平台。Songco-Casey等人结合联合收割机scRNA-seq和RNA FISH来表征章鱼视觉系统中的分子细胞类型。细胞类别由神经递质/神经肽和其他功能或发育标记物划定，揭示了视叶的亚层组织和对应于成熟细胞类型的未成熟神经元。
Cephalopods have a remarkable visual system, with a camera-type eye and high acuity vision that they use for a wide range of sophisticated visually driven behaviors. However, the cephalopod brain is organized dramatically differently from that of vertebrates and invertebrates, and beyond neuroanatomical descriptions, little is known regarding the cell types and molecular determinants of their visual system organization. Here, we present a comprehensive single-cell molecular atlas of the octopus optic lobe, which is the primary visual processing structure in the cephalopod brain. We combined single-cell RNA sequencing with RNA fluorescence in situ hybridization to both identify putative molecular cell types and determine their anatomical and spatial organization within the optic lobe. Our results reveal six major neuronal cell classes identified by neurotransmitter/neuropeptide usage, in addition to non-neuronal and immature neuronal populations. We find that additional markers divide these neuronal classes into subtypes with distinct anatomical localizations, revealing further diversity and a detailed laminar organization within the optic lobe. We also delineate the immature neurons within this continuously growing tissue into subtypes defined by evolutionarily conserved developmental genes as well as novel cephalopod- and octopus-specific genes. Together, these findings outline the organizational logic of the octopus visual system, based on functional determinants, laminar identity, and developmental markers/pathways. The resulting atlas presented here details the “parts list” for neural circuits used for vision in octopus, providing a platform for investigations into the development and function of the octopus visual system as well as the evolution of visual processing. Songco-Casey et al. combine scRNA-seq and RNA FISH to characterize molecular cell types in the octopus visual system. Cell classes are delineated by neurotransmitters/neuropeptides and additional functional or developmental markers, revealing both sublayer organization of the optic lobe and immature neurons that correspond to mature cell types.
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影响因子：
48
作者：
Cheng H;Concepcion GT;Feng X;Zhang H;Li H
通讯作者：
Li H
DOI：
10.1007/bf00217112
发表时间：
1991-01-01
期刊：
JOURNAL OF COMPARATIVE PHYSIOLOGY A-SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY
影响因子：
--
作者：
BULLOCK, TH;BUDELMANN, BU
通讯作者：
BUDELMANN, BU
影响因子：
2.9
作者：
CHICHERY, R;CHANELET, J
通讯作者：
CHANELET, J
影响因子：
5.3
作者：
BOYCOTT, BB;LETTVIN, JY;WALL, PD
通讯作者：
WALL, PD
影响因子：
7.7
作者：
Deryckere A;Styfhals R;Elagoz AM;Maes GE;Seuntjens E
通讯作者：
Seuntjens E