Assessing Basal and Acute Autophagic Responses in the Adult Drosophila Nervous System: The Impact of Gender, Genetics and Diet on Endogenous Pathway Profiles.
Assessing Basal and Acute Autophagic Responses in the Adult Drosophila Nervous System: The Impact of Gender, Genetics and Diet on Endogenous Pathway Profiles.
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DOI：
10.1371/journal.pone.0164239
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Finley KD
中科院分区：
文献类型：
--
作者：
Ratliff EP;Kotzebue RW;Molina B;Mauntz RE;Gonzalez A;Barekat A;El-Mecharrafie N;Garza S;Gurney MA;Achal M;Linton PJ;Harris GL;Finley KD
自噬途径对细胞和成年生物体的长期内稳态至关重要，通常在应激期间被激活。通路效率降低在几种进行性神经系统疾病中起着核心作用，这些疾病与细胞毒肽和蛋白质聚集体的积累有关。先前的研究表明，自噬途径的基因和转基因改变会影响成年果蝇的寿命和神经聚集特征。在这项研究中，我们已经确定了测量在成虫中枢神经系统中急性诱导自噬途径的方法。我们的发现表明，成年果蝇的基因、年龄和性别可以影响途径反应。此外，我们证明，暴露于间歇性禁食(IF)的中年雄性果蝇改善了神经元自噬的特征。与随意对照相比，接受IF处理的果蝇的神经聚集特征也更低，保持了更年轻的行为和更长的寿命。综上所述，我们提出了一种方法来检测成年果蝇中枢神经系统中发生的自噬特征的动态变化，以及一种新的IF治疗方案改善了老化神经系统的通路反应。
The autophagy pathway is critical for the long-term homeostasis of cells and adult organisms and is often activated during periods of stress. Reduced pathway efficacy plays a central role in several progressive neurological disorders that are associated with the accumulation of cytotoxic peptides and protein aggregates. Previous studies have shown that genetic and transgenic alterations to the autophagy pathway impacts longevity and neural aggregate profiles of adult Drosophila. In this study, we have identified methods to measure the acute in vivo induction of the autophagy pathway in the adult fly CNS. Our findings indicate that the genotype, age, and gender of adult flies can influence pathway responses. Further, we demonstrate that middle-aged male flies exposed to intermittent fasting (IF) had improved neuronal autophagic profiles. IF-treated flies also had lower neural aggregate profiles, maintained more youthful behaviors and longer lifespans, when compared to ad libitum controls. In summary, we present methodology to detect dynamic in vivo changes that occur to the autophagic profiles in the adult Drosophila CNS and that a novel IF-treatment protocol improves pathway response in the aging nervous system.
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影响因子：
5.3
作者：
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影响因子：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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