Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometry
Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometry
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通过流式细胞术评估隐甲藻脂质和 DHA 生产的低成本底物
DOI：
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Teresa Lopes da Silva
中科院分区：
文献类型：
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作者：
Tiago Taborda;Patrícia Moniz;A. Reis;Teresa Lopes da Silva
研究了隐甲藻（Crypthecodinium cohnii）在纯碳源（葡萄糖、乙酸盐、甘油）和低成本复合碳源（甘蔗糖蜜、粗甘油和食醋废液）上的生长情况，以生产油脂和DHA。在纯底物中，葡萄糖诱导最高的脂质含量（14.75%w/w DCW）和DHA含量（7.14 mg g −1 DCW）。在低成本底物中，粗甘油测定中观察到的脂质和DHA含量最高（分别为14.7%w/w DCW和6.56 mg g −1）。在所有研究的底物中，糖蜜诱导的DHA占总脂肪酸的比例最高（49.58%w/w TFA）。流式细胞仪分析表明，醋液诱导的C。cohnii细胞膜损伤（92.8%）。这些结果预示了在更大规模上使用这些低成本基底用于C的可能性。cohnii DHA和生物柴油生产，旨在实现零废物和降低工艺成本。
Crypthecodinium cohnii growth was studied on pure carbon sources (glucose, acetate, glycerol) and low-cost complex carbon sources (sugarcane molasses, crude glycerol and vinegar effluent) for lipid and DHA production. Among the pure substrates, glucose induced the highest lipid content (14.75% w/w DCW) and DHA content (7.14 mg g −1 DCW). Among the low-cost substrates, the highest lipid and DHA content were observed for the crude glycerol assay (14.7% w/w DCW and 6.56 mg g −1 , respectively). Molasses induced the highest proportion of DHA of total fatty acids (49.58% w/w TFA) among all the substrates studied. Flow cytometric analysis revealed that the vinegar effluent induced the highest proportion of C. cohnii cells with injured membrane (92.8%). These results foresee the possibility of using these low-cost substrates at a larger scale for C. cohnii DHA and biodiesel production, aiming at zero wastes and process costs reduction.
影响因子：
4.5
作者：
Hassan, Zahid;Sultana, Munawar;Westerhoff, Hans, V
通讯作者：
Westerhoff, Hans, V