Induction of primitive streak and Hensen's node by the posterior marginal zone in the early chick embryo.
Induction of primitive streak and Hensen's node by the posterior marginal zone in the early chick embryo.
复制标题
早期鸡胚后缘区诱导原条和亨森结。
作者：
R. Bachvarova;I. Skromne;C. Stern
在preprimitive streak鸡胚胎中，寻找一个能够诱导组织者的区域，相当于两栖动物胚胎的Nieuwkoop中心，集中在Koller的镰刀，下胚层和后边缘区。然而，没有明确的证据表明，诱导组织的诱导没有从诱导组织的贡献已提供任何这些结构。我们已经使用DiI/DiO标记来建立正常胚胎中Koller镰内和周围的中线细胞的命运。在上胚层中，形成条纹的细胞和不形成条纹的细胞之间的边界位于科勒镰刀的后缘，除了在X阶段，它位于上胚层中稍靠后的位置。下胚层和内胚层（在条纹下形成的第二层下层）在下层有不同的起源，并且goosecoid表达将它们区分开来。然后，我们使用鸡prestreak胚胎的前半部分作为受体的鹌鹑后边缘区的移植物，鹌鹑细胞可以识别随后与鹌鹑特异性抗体。前半部通常单独形成条纹，最常见的是从后缘。将不带Koller镰刀的鹌鹑后边缘区移植到前半部分的前侧。这些移植物能够显著增加X-XI期前半部前极出现条纹的频率，而不会导致条纹或结节。第十二阶段前半部分不再有反应。一个goosecoid表达的下胚层没有形成下诱导的条纹，表明它是不需要的条纹形成。我们的结论是，边缘区后科勒镰刀可以诱导条纹和节点，没有贡献的细胞诱导条纹。
In the preprimitive streak chick embryo, the search for a region capable of inducing the organizer, equivalent to the Nieuwkoop Center of the amphibian embryo, has focused on Koller's sickle, the hypoblast and the posterior marginal zone. However, no clear evidence for induction of an organizer without contribution from the inducing tissue has been provided for any of these structures. We have used DiI/DiO labeling to establish the fate of midline cells in and around Koller's sickle in the normal embryo. In the epiblast, the boundary between cells that contribute to the streak and those that do not lies at the posterior edge of Koller's sickle, except at stage X when it lies slightly more posteriorly in the epiblast. Hypoblast and endoblast (a second lower layer formed under the streak) have distinct origins in the lower layer, and goosecoid expression distinguishes between them. We then used anterior halves of chick prestreak embryos as recipients for grafts of quail posterior marginal zone; quail cells can be identified subsequently with a quail-specific antibody. Anterior halves alone usually formed a streak, most often from the posterior edge. Quail posterior marginal zones without Koller's sickle were grafted to the anterior side of anterior halves. These grafts were able to increase significantly the frequency of streaks arising from the anterior pole of stage X-XI anterior halves without contributing to the streak or node. Stage XII anterior halves no longer responded. A goosecoid-expressing hypoblast did not form under the induced streak, indicating that it is not required for streak formation. We conclude that the marginal zone posterior to Koller's sickle can induce a streak and node, without contributing cells to the induced streak.
脊索蛋白调节原条发育和诱导神经细胞的稳定性，但不足以用于鸡胚胎中的神经诱导。
DOI：
10.1242/dev.125.3.507
发表时间：
1998
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Streit,A;Lee,KJ;Woo,I;Roberts,C;Jessell,TM;Stern,CD
通讯作者：
Stern,CD
鸡胚脊索和底板发育的早期阶段由 HNF-3 β 的正常和诱导表达定义。
影响因子：
2.7
作者：
RuiziAltaba,A;Placzek,M;Baldassare,M;Dodd,J;Jessell,TM
通讯作者：
Jessell,TM